Substance P activates the release of histamine from human skin mast cells through a pertussis toxin-sensitive and protein kinase C-dependent mechanism

Michele Columbo, Edward M Horowitz, Anne Kagey-Sobotka, Lawrence M. Lichtenstein

Research output: Contribution to journalArticle

Abstract

Using pharmacologic agents, we explored the mechanism by which a potent neuropeptide, substance P, induces the secretion of histamine from human skin mast cells and compared their effects on substance P induced histamine release to the secretion activated by anti-IgE. Histamine release from human cutaneous mast cells induced by substance P was inhibited by the G(e)-protein inhibitor pertussis toxin that, in turn, did not affect the IgE-mediated secretion. Similarly to anti-IgE, two activators of protein kinase C, tetradecanoylphorbol acetate (TPA) and bryostatin 1, significantly inhibited the substance P-induced response. In contrast, drugs that enhance intracellular levels of cAMP, an inhibitor of protein kinases, genistein, and a protease inhibitor, AEBSF, did not affect substance P-induced histamine secretion, whereas these compounds significantly reduced the response initiated by anti-IgE. Our data demonstrate that substance P activates human cutaneous mast cells by acting on G proteins and protein kinase C. Our results also suggest that the biochemical pathways underlying mast cell activation by substance P and anti-IgE are to a great extent unrelated.

Original languageEnglish (US)
Pages (from-to)68-73
Number of pages6
JournalClinical Immunology and Immunopathology
Volume81
Issue number1
DOIs
StatePublished - Oct 1996

Fingerprint

Histamine Release
Pertussis Toxin
Substance P
Mast Cells
Protein Kinase C
Skin
Histamine
Genistein
Tetradecanoylphorbol Acetate
Protein Kinase Inhibitors
Protease Inhibitors
Neuropeptides
GTP-Binding Proteins
Immunoglobulin E
anti-IgE antibodies
Pharmaceutical Preparations
Proteins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Pathology and Forensic Medicine

Cite this

Substance P activates the release of histamine from human skin mast cells through a pertussis toxin-sensitive and protein kinase C-dependent mechanism. / Columbo, Michele; Horowitz, Edward M; Kagey-Sobotka, Anne; Lichtenstein, Lawrence M.

In: Clinical Immunology and Immunopathology, Vol. 81, No. 1, 10.1996, p. 68-73.

Research output: Contribution to journalArticle

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