TY - JOUR
T1 - Subretinal fluid stimulation of retinal pigment epithelial cell migration and proliferation is dependent on certain features of the detachment or its treatment
AU - Hackett, Sean F.
AU - Conway, Brian P.
AU - Campochiaro, Peter A.
PY - 1989/3
Y1 - 1989/3
N2 - Subretinal fluid was collected without contamination by blood by placing capillary tubes within drainage sclerotomies when retinal reattachment surgery was performed. All samples (N = 24) stimulated retinal pigment epithelial (RPE) cell migration and proliferation, but a wide range of activity existed (migration, 27.5% to 322.5% above baseline; proliferation, 7.5% to 318.3% above baseline). The correlation between migration- and proliferation-stimulating activity was not strong, suggesting the possibility that different agents are responsible for each. Several variables were examined for correlation with each activity, including age and sex of the patient; extent, duration, and height of the detachment; protein content of the fluid; and amount of cryopexy. It was found that proliferation-stimulating activity increased with the extent of detachment and duration of detachment, while migration-stimulating activity correlated best with amount of cryopexy given before drainage. These data suggest that retinal cryopexy enhances RPE cell migration activity in subretinal fluid, while RPE proliferation activity increases with the size and duration of detachment.
AB - Subretinal fluid was collected without contamination by blood by placing capillary tubes within drainage sclerotomies when retinal reattachment surgery was performed. All samples (N = 24) stimulated retinal pigment epithelial (RPE) cell migration and proliferation, but a wide range of activity existed (migration, 27.5% to 322.5% above baseline; proliferation, 7.5% to 318.3% above baseline). The correlation between migration- and proliferation-stimulating activity was not strong, suggesting the possibility that different agents are responsible for each. Several variables were examined for correlation with each activity, including age and sex of the patient; extent, duration, and height of the detachment; protein content of the fluid; and amount of cryopexy. It was found that proliferation-stimulating activity increased with the extent of detachment and duration of detachment, while migration-stimulating activity correlated best with amount of cryopexy given before drainage. These data suggest that retinal cryopexy enhances RPE cell migration activity in subretinal fluid, while RPE proliferation activity increases with the size and duration of detachment.
UR - http://www.scopus.com/inward/record.url?scp=0024535753&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024535753&partnerID=8YFLogxK
U2 - 10.1001/archopht.1989.01070010401033
DO - 10.1001/archopht.1989.01070010401033
M3 - Article
C2 - 2923564
AN - SCOPUS:0024535753
SN - 0003-9950
VL - 107
SP - 391
EP - 394
JO - Archives of ophthalmology
JF - Archives of ophthalmology
IS - 3
ER -