Sublethal oxygen-glucose deprivation alters hippocampal neuronal AMPA receptor expression and vulnerability to kainate-induced death

Howard S. Ying, Jochen H. Weishaupt, Margaret Grabb, Lorella M T Canzoniero, Stefano L. Sensi, Christian T. Sheline, Hannah Monyer, Dennis W. Choi

Research output: Contribution to journalArticle

Abstract

Recent studies have suggested that rats subjected to transient global brain ischemia develop depressed expression of GluR-B in CA1 hippocampal neurons. The present study was performed to determine whether a similar change in AMPA receptor expression could be triggered in vitro by sublethal oxygen-glucose deprivation in rat hippocampal neuronal cultures. mRNA was extracted from individual hippocampal neurons via patch electrodes and amplified by RT-PCR 24-48 hr after sublethal oxygen-glucose deprivation. Compared with controls, insulted neurons expressed increased levels of GluR- D flop. As an indication that this change in receptor expression was functionally significant, insulted cultures exhibited increased AMPA- or kainate-induced 45Ca2+ accumulation sensitive to Joro spider toxin and increased vulnerability to kainate-induced death. These data support the hypothesis that exposure to ischemia may enhance subsequent hippocampal neuronal vulnerability to AMPA receptor-mediated excitotoxicity by modifying the relative expression of AMPA receptor subunits in a manner that promotes Ca2+ permeability.

Original languageEnglish (US)
Pages (from-to)9536-9544
Number of pages9
JournalJournal of Neuroscience
Volume17
Issue number24
StatePublished - 1997
Externally publishedYes

Fingerprint

AMPA Receptors
Kainic Acid
Oxygen
Neurons
Glucose
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Brain Ischemia
Permeability
Electrodes
Ischemia
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Calcium accumulation
  • GluR-D
  • Glutamate receptor regulation
  • Ischemia
  • Neuronal vulnerability
  • Single cell RT-PCR

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ying, H. S., Weishaupt, J. H., Grabb, M., Canzoniero, L. M. T., Sensi, S. L., Sheline, C. T., ... Choi, D. W. (1997). Sublethal oxygen-glucose deprivation alters hippocampal neuronal AMPA receptor expression and vulnerability to kainate-induced death. Journal of Neuroscience, 17(24), 9536-9544.

Sublethal oxygen-glucose deprivation alters hippocampal neuronal AMPA receptor expression and vulnerability to kainate-induced death. / Ying, Howard S.; Weishaupt, Jochen H.; Grabb, Margaret; Canzoniero, Lorella M T; Sensi, Stefano L.; Sheline, Christian T.; Monyer, Hannah; Choi, Dennis W.

In: Journal of Neuroscience, Vol. 17, No. 24, 1997, p. 9536-9544.

Research output: Contribution to journalArticle

Ying, HS, Weishaupt, JH, Grabb, M, Canzoniero, LMT, Sensi, SL, Sheline, CT, Monyer, H & Choi, DW 1997, 'Sublethal oxygen-glucose deprivation alters hippocampal neuronal AMPA receptor expression and vulnerability to kainate-induced death', Journal of Neuroscience, vol. 17, no. 24, pp. 9536-9544.
Ying, Howard S. ; Weishaupt, Jochen H. ; Grabb, Margaret ; Canzoniero, Lorella M T ; Sensi, Stefano L. ; Sheline, Christian T. ; Monyer, Hannah ; Choi, Dennis W. / Sublethal oxygen-glucose deprivation alters hippocampal neuronal AMPA receptor expression and vulnerability to kainate-induced death. In: Journal of Neuroscience. 1997 ; Vol. 17, No. 24. pp. 9536-9544.
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