Subconjunctival delivery of antibiotics in a controlled-release system: A novel anti-infective prophylaxis approach for cataract surgery

José A. Cardillo, Fernando Paganelli, Luiz A.S. Melo, Arnobio A. Silva, Antonio C. Pizzolitto, Anselmo G. Oliveira, Rubens Belfort, Rogerio A. Costa, Cristina M. Franzini, Baruch D. Kuppermann, Acacio A.S. Lima-Filho, Daniel Lavinsky, Ana L. Höfling-Lima, Quan Dong Nguyen, Silvana A. Schellini, Mirian Skaf

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To compare the efficacy of subconjunctival injection of a combination of triamcinolone and ciprofloxacin hydrochloride, 2 mg/0.1 mL, in a controlledrelease system (DuoCat) with that of ciprofloxacin hydrochloride, 0.3%, eyedrops for infection prophylaxis. Methods: Rabbit eyes were injected subconjunctivally with a combination of triamcinolone and ciprofloxacin hydrochloride, 2 mg/0.1 mL, or ciprofloxacin hydrochloride, 2 mg/0.1 mL, alone. The aqueous and vitreous humor pharmacokinetic profiles were compared with those of a single drop of ciprofloxacin hydrochloride, 0.3%, 6 times daily. In 45 rabbits, Staphylococcus aureus was injected into the anterior chamber: 15 randomly received 1 drop of ciprofloxacin hydrochloride, 0.3%, every 4 hours during 24 hours; 15 received drops of balanced salt solution; and 15 received a combination of triamcinolone and ciprofloxacin hydrochloride, 2 mg/0.1 mL. After 24 hours, endophthalmitis scores were recorded, aqueous and vitreous humors underwent culture, and histologic analysis was performed. Results: The combined triamcinolone and ciprofloxacin treatment allowed higher intraocular levels of ciprofloxacin. The median endophthalmitis clinical scores for the combination of triamcinolone and ciprofloxacin and ciprofloxacin-only eyedrop groups were equivalent (P=.42) and were significantly lower than those of the balanced salt solution group (P<.001). The culture was negative for S aureus in the combined triamcinolone and ciprofloxacin and ciprofloxacin eyedrop regimens. No adverse effects were observed with either route. Conclusions: Ciprofloxacin eyedrops and combined triamcinolone and ciprofloxacin were equally tolerated and efficacious. The combined triamcinolone and ciprofloxacin treatment may eliminate noncompliance issues and may prove to be a valuable clinical tool for surgical prophylaxis. Clinical Relevance: The combined triamcinolone and ciprofloxacin treatment may be a new useful strategy for surgical prophylaxis.

Original languageEnglish (US)
Pages (from-to)81-87
Number of pages7
JournalArchives of ophthalmology
Volume128
Issue number1
DOIs
StatePublished - Jan 2010

ASJC Scopus subject areas

  • Ophthalmology

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