Subcellular optogenetic stimulation platform for studying activity-dependent axon myelination in vitro

Agata Blasiak, Sudip Nag, In Hong Yang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Activity-dependent myelination modulates neuron conduction velocity and as such it is essential for a correct wiring of a whole nervous system. Increasing myelination through inducing neuron activity has been proposed as a treatment strategy for demyelination diseases. Yet, the mechanisms and the effects of activity-dependent myelination remain elusive—new tools are needed. In this chapter, we describe a novel compartmentalized device integrated with an optogenetic stimulator for studying activity-dependent myelination in vitro. The platform can be modified to include multiple cell types, stimulation modes, and experimental readouts to answer a specific research question. This versatility combined with a precise control over spatial extent of the stimulation and the stimulation pattern make the proposed platform a valuable tool for molecular myelination studies.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages207-224
Number of pages18
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1791
ISSN (Print)1064-3745

Keywords

  • Activity-dependent myelination
  • Compartmentalized culture
  • In vitro myelination
  • Microfluidics
  • Neurons
  • Oligodendrocytes
  • Optogenetics
  • Subcellular stimulation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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