Subcellular localization of patched and smoothened, the receptors for sonic hedgehog signaling, in the hippocampal neuron

Ronald S. Petralia, Catherine M. Schwartz, Ya Xian Wang, Mark P. Mattson, Pamela J. Yao

Research output: Contribution to journalArticle

Abstract

Cumulative evidence suggests that, aside from patterning the embryonic neural tube, Sonic hedgehog (Shh) signaling plays important roles in the mature nervous system. In this study, we investigate the expression and localization of the Shh signaling receptors, Patched (Ptch) and Smoothened (Smo), in the hippocampal neurons of young and mature rats. Reverse transcriptase-polymerase chain reaction and immunoblotting analyses show that the expression of Ptch and Smo remains at a moderate level in young postnatal and adult brains. By using immunofluorescence light microscopy and immunoelectron microscopy, we examine the spatial distribution of Ptch and Smo within the hippocampal neurons. In young developing neurons, Ptch and Smo are present in the processes and are clustered at their growth cones. In mature neurons, Ptch and Smo are concentrated in dendrites, spines, and postsynaptic sites. Synaptic Ptch and Smo often co-exist with unusual structures-synaptic spinules and autophagosomes. Our results reveal the anatomical organization of the Shh receptors within both the young and the mature hippocampal neurons.

Original languageEnglish (US)
Pages (from-to)3684-3699
Number of pages16
JournalJournal of Comparative Neurology
Volume519
Issue number18
DOIs
StatePublished - Dec 15 2011
Externally publishedYes

Fingerprint

Hedgehogs
Neurons
Growth Cones
Neural Tube
Immunoelectron Microscopy
Dendrites
Reverse Transcriptase Polymerase Chain Reaction
Fluorescence Microscopy
Immunoblotting
Nervous System
Young Adult
Spine
Smoothened Receptor
Patched Receptors
Light
Brain

Keywords

  • Autophagosome
  • Hippocampal neuron
  • Patched
  • Primary cilium
  • Smoothened
  • Sonic hedgehog
  • Synapse
  • Synaptic spinule

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Subcellular localization of patched and smoothened, the receptors for sonic hedgehog signaling, in the hippocampal neuron. / Petralia, Ronald S.; Schwartz, Catherine M.; Wang, Ya Xian; Mattson, Mark P.; Yao, Pamela J.

In: Journal of Comparative Neurology, Vol. 519, No. 18, 15.12.2011, p. 3684-3699.

Research output: Contribution to journalArticle

Petralia, Ronald S. ; Schwartz, Catherine M. ; Wang, Ya Xian ; Mattson, Mark P. ; Yao, Pamela J. / Subcellular localization of patched and smoothened, the receptors for sonic hedgehog signaling, in the hippocampal neuron. In: Journal of Comparative Neurology. 2011 ; Vol. 519, No. 18. pp. 3684-3699.
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