Subcellular distribution in cerebral cortex of two proteins phosphorylated by a cAMP-dependent protein kinase

T. Ueda, P. Greengard, K. Berzins, R. S. Cohen, F. Blomberg, D. J. Grab, P. Siekevitz

Research output: Contribution to journalArticle

Abstract

The subcellular distribution of Proteins Ia and Ib, two proteins which serve as specific substrates for protein kinases present in mammalian brain, was studied in the dog cerebral cortex. Proteins Ia and Ib were found to be most highly enriched in synaptic vesicle fractions; they were also present in postsynaptic density and synaptic membrane fractions in significant amounts. Proteins Ia and Ib present in the synaptic vesicle fraction appear to be similar, if not identical, to those present in the postsynaptic density fraction as judged by several criteria: (a) the ability to serve as substrate for cAMP-dependent protein kinase, (b) electrophoretic mobility in the presence of sodium dodecyl sulfate, (c) extractability with NH 4Cl or EGTA, and (d) fragmentation to electrophoretically similar peptides by a purified Staphylococcus aureus protease. In addition, the postsynaptic density fraction has been found to contain cAMP-dependent Protein Ia and Protein Ib kinase activity. The subcellular localization of Proteins Ia and Ib suggests a role for these proteins in the physiology of the synapse.

Original languageEnglish (US)
Pages (from-to)308-319
Number of pages12
JournalJournal of Cell Biology
Volume83
Issue number2 I
DOIs
StatePublished - 1979
Externally publishedYes

Fingerprint

Cyclic AMP-Dependent Protein Kinases
Cerebral Cortex
Post-Synaptic Density
Proteins
Synaptic Vesicles
Protein Kinases
Synaptic Membranes
Egtazic Acid
Sodium Dodecyl Sulfate
Synapses
Staphylococcus aureus
Peptide Hydrolases
Dogs
Peptides
Brain

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ueda, T., Greengard, P., Berzins, K., Cohen, R. S., Blomberg, F., Grab, D. J., & Siekevitz, P. (1979). Subcellular distribution in cerebral cortex of two proteins phosphorylated by a cAMP-dependent protein kinase. Journal of Cell Biology, 83(2 I), 308-319. https://doi.org/10.1083/jcb.83.2.308

Subcellular distribution in cerebral cortex of two proteins phosphorylated by a cAMP-dependent protein kinase. / Ueda, T.; Greengard, P.; Berzins, K.; Cohen, R. S.; Blomberg, F.; Grab, D. J.; Siekevitz, P.

In: Journal of Cell Biology, Vol. 83, No. 2 I, 1979, p. 308-319.

Research output: Contribution to journalArticle

Ueda, T, Greengard, P, Berzins, K, Cohen, RS, Blomberg, F, Grab, DJ & Siekevitz, P 1979, 'Subcellular distribution in cerebral cortex of two proteins phosphorylated by a cAMP-dependent protein kinase', Journal of Cell Biology, vol. 83, no. 2 I, pp. 308-319. https://doi.org/10.1083/jcb.83.2.308
Ueda, T. ; Greengard, P. ; Berzins, K. ; Cohen, R. S. ; Blomberg, F. ; Grab, D. J. ; Siekevitz, P. / Subcellular distribution in cerebral cortex of two proteins phosphorylated by a cAMP-dependent protein kinase. In: Journal of Cell Biology. 1979 ; Vol. 83, No. 2 I. pp. 308-319.
@article{a832a2b70ff644069ea8185e27e3883c,
title = "Subcellular distribution in cerebral cortex of two proteins phosphorylated by a cAMP-dependent protein kinase",
abstract = "The subcellular distribution of Proteins Ia and Ib, two proteins which serve as specific substrates for protein kinases present in mammalian brain, was studied in the dog cerebral cortex. Proteins Ia and Ib were found to be most highly enriched in synaptic vesicle fractions; they were also present in postsynaptic density and synaptic membrane fractions in significant amounts. Proteins Ia and Ib present in the synaptic vesicle fraction appear to be similar, if not identical, to those present in the postsynaptic density fraction as judged by several criteria: (a) the ability to serve as substrate for cAMP-dependent protein kinase, (b) electrophoretic mobility in the presence of sodium dodecyl sulfate, (c) extractability with NH 4Cl or EGTA, and (d) fragmentation to electrophoretically similar peptides by a purified Staphylococcus aureus protease. In addition, the postsynaptic density fraction has been found to contain cAMP-dependent Protein Ia and Protein Ib kinase activity. The subcellular localization of Proteins Ia and Ib suggests a role for these proteins in the physiology of the synapse.",
author = "T. Ueda and P. Greengard and K. Berzins and Cohen, {R. S.} and F. Blomberg and Grab, {D. J.} and P. Siekevitz",
year = "1979",
doi = "10.1083/jcb.83.2.308",
language = "English (US)",
volume = "83",
pages = "308--319",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "2 I",

}

TY - JOUR

T1 - Subcellular distribution in cerebral cortex of two proteins phosphorylated by a cAMP-dependent protein kinase

AU - Ueda, T.

AU - Greengard, P.

AU - Berzins, K.

AU - Cohen, R. S.

AU - Blomberg, F.

AU - Grab, D. J.

AU - Siekevitz, P.

PY - 1979

Y1 - 1979

N2 - The subcellular distribution of Proteins Ia and Ib, two proteins which serve as specific substrates for protein kinases present in mammalian brain, was studied in the dog cerebral cortex. Proteins Ia and Ib were found to be most highly enriched in synaptic vesicle fractions; they were also present in postsynaptic density and synaptic membrane fractions in significant amounts. Proteins Ia and Ib present in the synaptic vesicle fraction appear to be similar, if not identical, to those present in the postsynaptic density fraction as judged by several criteria: (a) the ability to serve as substrate for cAMP-dependent protein kinase, (b) electrophoretic mobility in the presence of sodium dodecyl sulfate, (c) extractability with NH 4Cl or EGTA, and (d) fragmentation to electrophoretically similar peptides by a purified Staphylococcus aureus protease. In addition, the postsynaptic density fraction has been found to contain cAMP-dependent Protein Ia and Protein Ib kinase activity. The subcellular localization of Proteins Ia and Ib suggests a role for these proteins in the physiology of the synapse.

AB - The subcellular distribution of Proteins Ia and Ib, two proteins which serve as specific substrates for protein kinases present in mammalian brain, was studied in the dog cerebral cortex. Proteins Ia and Ib were found to be most highly enriched in synaptic vesicle fractions; they were also present in postsynaptic density and synaptic membrane fractions in significant amounts. Proteins Ia and Ib present in the synaptic vesicle fraction appear to be similar, if not identical, to those present in the postsynaptic density fraction as judged by several criteria: (a) the ability to serve as substrate for cAMP-dependent protein kinase, (b) electrophoretic mobility in the presence of sodium dodecyl sulfate, (c) extractability with NH 4Cl or EGTA, and (d) fragmentation to electrophoretically similar peptides by a purified Staphylococcus aureus protease. In addition, the postsynaptic density fraction has been found to contain cAMP-dependent Protein Ia and Protein Ib kinase activity. The subcellular localization of Proteins Ia and Ib suggests a role for these proteins in the physiology of the synapse.

UR - http://www.scopus.com/inward/record.url?scp=0018608035&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018608035&partnerID=8YFLogxK

U2 - 10.1083/jcb.83.2.308

DO - 10.1083/jcb.83.2.308

M3 - Article

C2 - 227912

AN - SCOPUS:0018608035

VL - 83

SP - 308

EP - 319

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 2 I

ER -