Study on the selective restriction of mass transport during isoelectric focusing

We separated the electrode solutions by Nafion® membranes, which selectively restrict the processes of migration and diffusion in the whole electrophoretic system. Under these newly developed conditions the ampholyte pH gradient is strongly affected, behaving as a carrier with a very low ionic strength, in which the magnitude of the current flowing through the system rapidly decreases. Thus the isoelectric focusing can be carried out closer to steady state, obtaining sharp protein separation for a considerably shorter period of time.