Study of the multiple endocrine neoplasia type 1, growth hormone-releasing hormone receptor, Gsα, and Gi2α genes in isolated familial acromegaly

Beatriz H. Jorge, Sunita K. Agarwal, Valéria S. Lando, Roberto Salvatori, Ruth R. Barbero, Neusa Abelin, Michael A. Levine, Stephen J. Marx, Sergio P.A. Toledo

Research output: Contribution to journalArticle


Familial acromegaly may occur as an isolated pituitary disorder or as a feature of hereditary syndromes, such as multiple endocrine neoplasia type 1 (MEN1) or the Carney complex. Herein, we characterized a newly identified kindred with isolated acromegaly and searched for germline mutation in genes that have been associated with endocrine tumors [i.e. MEN1, Gsα (GNAS1), and Gi2α (GNAI2)], as well as the GHRH receptor (GHRH-R) gene. Genomic DNA was used to amplify exons 2-10 of MEN1, followed by dideoxy fingerprinting mutation analysis and direct sequencing. The GHRH-R gene was analyzed via direct sequencing of PCR-amplified fragments representing the coding exons and intron-exon junctions. To exclude mutation at hot spot areas of GNAS1 and GNAI2, exons 8 and 9 of GNAS1 and exons 5 and 6 of GNAI2 were amplified and screened for mutation via denaturing gradient gel electrophoresis. No mutations were detected in any of the four genes. The present data extend prior reports of the absence of mutation in MEN1, GHRH-R, and GNAS1 and describe the first family with isolated acromegaly in which germline mutation in GNAI2 has been searched.

Original languageEnglish (US)
Pages (from-to)542-544
Number of pages3
JournalJournal of Clinical Endocrinology and Metabolism
Issue number2
StatePublished - Mar 22 2001


ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Endocrinology
  • Clinical Biochemistry
  • Biochemistry, medical

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