Study of relapsing remitting experimental allergic encephalomyelitis SJL mouse model using MION-46L enhanced in vivo MRI: Early histopathological correlation

Su Xu, E. Kay Jordan, Stefan Brocke, Jeff W.M. Bulte, Laura Quigley, Nancy Tresser, John L. Ostuni, Yihong Yang, Henry F. McFarland, Joseph A. Frank

Research output: Contribution to journalArticle

Abstract

MION-46L, a superparamagnetic iron oxide contrast agent, was investigated for its ability to increase the sensitivity of in vivo 3D MRI in the detection of brain lesions in a chronic experimental allergic encephalomyelitis (crEAE) mouse model. Lesion conspicuity on postcontrast 3D MRI was dramatically enhanced as compared to precontrast images corresponding to areas of inflammatory and demyelinating lesions. MION-46L could be detected on Prussian blue iron stain in the vascular endothelium, the perivascular space, and in macrophages within perivascular cuffs and areas of inflammation and demyelination. By taking advantage of the MION-46L induced macroscopic susceptibility effect, acute early lesions measuring only 100 μm in diameter could be detected. MION-46L enhanced MRI may be used to 1) provide a unique sensitivity in EAE lesion detection and correlate imaging to histopathology; 2) help to understand EAE lesion development and its underlying pathophysiology; and 3) eventually assist in preclinical screening of new experimental therapies directed at patients with multiple sclerosis (MS).

Original languageEnglish (US)
Pages (from-to)549-558
Number of pages10
JournalJournal of neuroscience research
Volume52
Issue number5
DOIs
StatePublished - Jun 1 1998
Externally publishedYes

Keywords

  • Contrast agent enhanced
  • EAE mouse
  • In vivo MRI
  • MOIN-46L iron oxide

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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