Study of protein characteristics that influence entry into the cerebrospinal fluid of normal mice and mice with encephalitis

Diane Griffin, J. Giffels

Research output: Contribution to journalArticle

Abstract

Entry of protein into the cerebrospinal fluid (CSF) from the blood is partially determined by the size of the protein. To determine whether other characteristics of proteins influence CSF entry, proteins or protein fragments were iodinated, inoculated intravenously, and serum and CSF were sampled at later times. The Fc fragment of immunoglobulin G (IgG) did not enter the CSF significantly better than the Fab fragment suggesting that choroidal Fc receptors are not of importance for selective immunoglobulin entry. To determine the role of protein charge on entry, bovine serum albumin [isolelectric point (pI) = 3.9] was chemically altered to provide an albumin with an average pI of 6 (A-6) and another with a pI of 8.5 (A-8). All albumins were of the same size on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A-8 entered the CSF ~10-fold better than the native albumin. A-6 was intermediate, entering approximately twofold better. At the time of increased CSF protein concentration during an acute viral encephalitis these differences were narrowed but not eliminated. It is concluded that charge is an important determinant of protein entry into the CSF.

Original languageEnglish (US)
Pages (from-to)289-295
Number of pages7
JournalJournal of Clinical Investigation
Volume70
Issue number2
StatePublished - 1982

Fingerprint

Encephalitis
Cerebrospinal Fluid
Cerebrospinal Fluid Proteins
Albumins
Proteins
Viral Encephalitis
Immunoglobulin Fc Fragments
Immunoglobulin Fab Fragments
Fc Receptors
Bovine Serum Albumin
Sodium Dodecyl Sulfate
Immunoglobulins
Polyacrylamide Gel Electrophoresis
Immunoglobulin G
Serum

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{a90913a7625646a2a34819e076a7926e,
title = "Study of protein characteristics that influence entry into the cerebrospinal fluid of normal mice and mice with encephalitis",
abstract = "Entry of protein into the cerebrospinal fluid (CSF) from the blood is partially determined by the size of the protein. To determine whether other characteristics of proteins influence CSF entry, proteins or protein fragments were iodinated, inoculated intravenously, and serum and CSF were sampled at later times. The Fc fragment of immunoglobulin G (IgG) did not enter the CSF significantly better than the Fab fragment suggesting that choroidal Fc receptors are not of importance for selective immunoglobulin entry. To determine the role of protein charge on entry, bovine serum albumin [isolelectric point (pI) = 3.9] was chemically altered to provide an albumin with an average pI of 6 (A-6) and another with a pI of 8.5 (A-8). All albumins were of the same size on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A-8 entered the CSF ~10-fold better than the native albumin. A-6 was intermediate, entering approximately twofold better. At the time of increased CSF protein concentration during an acute viral encephalitis these differences were narrowed but not eliminated. It is concluded that charge is an important determinant of protein entry into the CSF.",
author = "Diane Griffin and J. Giffels",
year = "1982",
language = "English (US)",
volume = "70",
pages = "289--295",
journal = "Journal of Clinical Investigation",
issn = "0021-9738",
publisher = "The American Society for Clinical Investigation",
number = "2",

}

TY - JOUR

T1 - Study of protein characteristics that influence entry into the cerebrospinal fluid of normal mice and mice with encephalitis

AU - Griffin, Diane

AU - Giffels, J.

PY - 1982

Y1 - 1982

N2 - Entry of protein into the cerebrospinal fluid (CSF) from the blood is partially determined by the size of the protein. To determine whether other characteristics of proteins influence CSF entry, proteins or protein fragments were iodinated, inoculated intravenously, and serum and CSF were sampled at later times. The Fc fragment of immunoglobulin G (IgG) did not enter the CSF significantly better than the Fab fragment suggesting that choroidal Fc receptors are not of importance for selective immunoglobulin entry. To determine the role of protein charge on entry, bovine serum albumin [isolelectric point (pI) = 3.9] was chemically altered to provide an albumin with an average pI of 6 (A-6) and another with a pI of 8.5 (A-8). All albumins were of the same size on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A-8 entered the CSF ~10-fold better than the native albumin. A-6 was intermediate, entering approximately twofold better. At the time of increased CSF protein concentration during an acute viral encephalitis these differences were narrowed but not eliminated. It is concluded that charge is an important determinant of protein entry into the CSF.

AB - Entry of protein into the cerebrospinal fluid (CSF) from the blood is partially determined by the size of the protein. To determine whether other characteristics of proteins influence CSF entry, proteins or protein fragments were iodinated, inoculated intravenously, and serum and CSF were sampled at later times. The Fc fragment of immunoglobulin G (IgG) did not enter the CSF significantly better than the Fab fragment suggesting that choroidal Fc receptors are not of importance for selective immunoglobulin entry. To determine the role of protein charge on entry, bovine serum albumin [isolelectric point (pI) = 3.9] was chemically altered to provide an albumin with an average pI of 6 (A-6) and another with a pI of 8.5 (A-8). All albumins were of the same size on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. A-8 entered the CSF ~10-fold better than the native albumin. A-6 was intermediate, entering approximately twofold better. At the time of increased CSF protein concentration during an acute viral encephalitis these differences were narrowed but not eliminated. It is concluded that charge is an important determinant of protein entry into the CSF.

UR - http://www.scopus.com/inward/record.url?scp=0019997260&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019997260&partnerID=8YFLogxK

M3 - Article

C2 - 7096568

AN - SCOPUS:0019997260

VL - 70

SP - 289

EP - 295

JO - Journal of Clinical Investigation

JF - Journal of Clinical Investigation

SN - 0021-9738

IS - 2

ER -