Study of phosphotyrosine containing peptides by atmospheric pressure maldi (AP MALDI) quadrupole ion trap mass spectrometry

Susanne C. Moyer; Amina S. Woods; Robert J. Cotter

Study of phosphotyrosine containing peptides by atmospheric pressure maldi (AP MALDI) quadrupole ion trap mass spectrometry

Susanne C. Moyer, Amina S. Woods, Robert J. Cotter

Research output: Contribution to conference › Paper › peer-review

Abstract

The phosphotyrosine containing peptides were studied by using infrared atmospheric pressure-matrix assisted laser desorption/ionization(IR AP-MALDI) ion trap mass spectrometers (ITMS). The computational method to aid in the determination of the types of interactions that could lead to gas phase dephosphorylation of phosphotyrosine were also utilized for the study. It was found that the peptides containing arginine or lysine residues lost 98 Da under collision induced dissociation (CID) conditions and peptides without lysine residues did not loss 98 Da under CID conditions. The results show that the cation-π interaction facilitate specific fragmentation of the phosphate group from phosphotyrosine under CID conditions.

Original language	English (US)
Pages	391-392
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

Study of phosphotyrosine containing peptides by atmospheric pressure maldi (AP MALDI) quadrupole ion trap mass spectrometry. / Moyer, Susanne C.; Woods, Amina S.; Cotter, Robert J.
2002. 391-392 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

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title = "Study of phosphotyrosine containing peptides by atmospheric pressure maldi (AP MALDI) quadrupole ion trap mass spectrometry",

abstract = "The phosphotyrosine containing peptides were studied by using infrared atmospheric pressure-matrix assisted laser desorption/ionization(IR AP-MALDI) ion trap mass spectrometers (ITMS). The computational method to aid in the determination of the types of interactions that could lead to gas phase dephosphorylation of phosphotyrosine were also utilized for the study. It was found that the peptides containing arginine or lysine residues lost 98 Da under collision induced dissociation (CID) conditions and peptides without lysine residues did not loss 98 Da under CID conditions. The results show that the cation-π interaction facilitate specific fragmentation of the phosphate group from phosphotyrosine under CID conditions.",

author = "Moyer, {Susanne C.} and Woods, {Amina S.} and Cotter, {Robert J.}",

year = "2002",

language = "English (US)",

pages = "391--392",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

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TY - CONF

T1 - Study of phosphotyrosine containing peptides by atmospheric pressure maldi (AP MALDI) quadrupole ion trap mass spectrometry

AU - Moyer, Susanne C.

AU - Woods, Amina S.

AU - Cotter, Robert J.

PY - 2002

Y1 - 2002

N2 - The phosphotyrosine containing peptides were studied by using infrared atmospheric pressure-matrix assisted laser desorption/ionization(IR AP-MALDI) ion trap mass spectrometers (ITMS). The computational method to aid in the determination of the types of interactions that could lead to gas phase dephosphorylation of phosphotyrosine were also utilized for the study. It was found that the peptides containing arginine or lysine residues lost 98 Da under collision induced dissociation (CID) conditions and peptides without lysine residues did not loss 98 Da under CID conditions. The results show that the cation-π interaction facilitate specific fragmentation of the phosphate group from phosphotyrosine under CID conditions.

AB - The phosphotyrosine containing peptides were studied by using infrared atmospheric pressure-matrix assisted laser desorption/ionization(IR AP-MALDI) ion trap mass spectrometers (ITMS). The computational method to aid in the determination of the types of interactions that could lead to gas phase dephosphorylation of phosphotyrosine were also utilized for the study. It was found that the peptides containing arginine or lysine residues lost 98 Da under collision induced dissociation (CID) conditions and peptides without lysine residues did not loss 98 Da under CID conditions. The results show that the cation-π interaction facilitate specific fragmentation of the phosphate group from phosphotyrosine under CID conditions.

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M3 - Paper

AN - SCOPUS:2442715464

SP - 391

EP - 392

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Study of phosphotyrosine containing peptides by atmospheric pressure maldi (AP MALDI) quadrupole ion trap mass spectrometry

Abstract

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