Study of oxidative-stress in rotavirus infected infant mice

Chhinder Sodhi, R. Katyal, S. V. Rana, S. Attri, V. Singh

Research output: Contribution to journalArticle

Abstract

Rotavirus induced diarrhoea was investigated in neonatal mice. Assessment of oxidative/antioxidative profile was the mechanistic approach to study the nature of injury. Neonatal mice (NMRI strain) were infected orally with the homologous strain of (EB) rotavirus (serotype 3). The peak severity of rotavirus infection was attained on the third day post infection. The whole small intestine of neonatal mice on day 3 post infection was homogenized and analysed for oxidative/antioxidative profile. Glutathione and related thiols were significantly declined in rotavirus infected group. Superoxide dismutase, glutathione peroxidase and glutathione-S-transferase (1-chloro 2, 4 dinitrobenzene) activities were also decreased in the rotavirus infected group. The activities of glutathione reductase and glutathione-S-transferase (ethacrynic acid) however were elevated with rotavirus infection in comparison to the control group. Similarly, ADP-FeCl3, NADPH induced lipid peroxidation was elevated with rotavirus infection. Thus the altered oxidative/antioxidative profile indicated the presence of oxidative stress in the rotavirus infected group and can be postulated to have a prominent role in the pathogenesis of the disease.

Original languageEnglish (US)
Pages (from-to)245-249
Number of pages5
JournalIndian Journal of Medical Research
Volume104
Issue numberOCT
StatePublished - Oct 1996
Externally publishedYes

Fingerprint

Oxidative stress
Rotavirus
Glutathione Transferase
Oxidative Stress
Rotavirus Infections
Ethacrynic Acid
Dinitrochlorobenzene
Glutathione Reductase
Glutathione Peroxidase
NADP
Sulfhydryl Compounds
Adenosine Diphosphate
Superoxide Dismutase
Glutathione
Lipids
Infection
Lipid Peroxidation
Small Intestine
Diarrhea
Control Groups

Keywords

  • Antioxidative enzymes
  • Diarrhoea
  • Oxidative stress
  • Rotavirus

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Sodhi, C., Katyal, R., Rana, S. V., Attri, S., & Singh, V. (1996). Study of oxidative-stress in rotavirus infected infant mice. Indian Journal of Medical Research, 104(OCT), 245-249.

Study of oxidative-stress in rotavirus infected infant mice. / Sodhi, Chhinder; Katyal, R.; Rana, S. V.; Attri, S.; Singh, V.

In: Indian Journal of Medical Research, Vol. 104, No. OCT, 10.1996, p. 245-249.

Research output: Contribution to journalArticle

Sodhi, C, Katyal, R, Rana, SV, Attri, S & Singh, V 1996, 'Study of oxidative-stress in rotavirus infected infant mice', Indian Journal of Medical Research, vol. 104, no. OCT, pp. 245-249.
Sodhi C, Katyal R, Rana SV, Attri S, Singh V. Study of oxidative-stress in rotavirus infected infant mice. Indian Journal of Medical Research. 1996 Oct;104(OCT):245-249.
Sodhi, Chhinder ; Katyal, R. ; Rana, S. V. ; Attri, S. ; Singh, V. / Study of oxidative-stress in rotavirus infected infant mice. In: Indian Journal of Medical Research. 1996 ; Vol. 104, No. OCT. pp. 245-249.
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