Study of oxidative-stress in rifampicin-induced hepatic injury in growing rats with and without protein-energy malnutrition

Chhinder Sodhi, Satyavati Rana, Saroj Mehta, Kim Vaiphei, Ramesh Chand Goel, Satish Kumar Mehta

Research output: Contribution to journalArticle

Abstract

1. Rifampicin (RMP) induced hepatic injury was investigated in growing rats. The interaction of moderate and severe protein-energy malnutrition (PEM) was also investigated. 2. Status of oxidative/antioxidative profile was studied by the mechanistic approach, to enumerate the nature of injury. 3. Successful hepatic injury in rats was produced by giving intraperitoneal injection of RMP (50 mg/kg/day). 4. Hepatic lipid peroxidation was significantly increased in all the RMP treated rats. 5. Superoxide dismutase, catalase and glutathione peroxidase activities in the hepatic tissue decreased with RMP treatment. 6. Hepatic thiols represented as total and protein-bound thiols, showed significant elevation, whereas the non protein thiols remain unchanged with RMP treatment. 7. Glutathione-S-transferases also showed significant elevation against 1,2-dichloro-4 nitrobenzene (DCNB) and ethacrynic acid (EA) as substrates. 8. The oxidative/antioxidative profile was observed to be more severely affected with coexistence of malnutrition. 9. Histopathological correlation showed an additional fatty infiltration of hepatocytes with coexistence of malnutrition. 10. Thus, in conclusion, it can be speculated that an altered oxidative/antioxidative profile is the closely associated with production of RMP induced hepatic injury.

Original languageEnglish (US)
Pages (from-to)315-321
Number of pages7
JournalHuman and Experimental Toxicology
Volume16
Issue number6
StatePublished - Jun 1997
Externally publishedYes

Fingerprint

Protein-Energy Malnutrition
Oxidative stress
Rifampin
Rats
Oxidative Stress
Liver
Wounds and Injuries
Sulfhydryl Compounds
Proteins
Malnutrition
Ethacrynic Acid
Glutathione Peroxidase
Glutathione Transferase
Intraperitoneal Injections
Infiltration
Catalase
Lipid Peroxidation
Superoxide Dismutase
Hepatocytes
Tissue

Keywords

  • Hepatotoxicity
  • Oxidative-stress
  • Protein-energy malnutrition
  • Rifampicin

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Study of oxidative-stress in rifampicin-induced hepatic injury in growing rats with and without protein-energy malnutrition. / Sodhi, Chhinder; Rana, Satyavati; Mehta, Saroj; Vaiphei, Kim; Goel, Ramesh Chand; Mehta, Satish Kumar.

In: Human and Experimental Toxicology, Vol. 16, No. 6, 06.1997, p. 315-321.

Research output: Contribution to journalArticle

Sodhi, Chhinder ; Rana, Satyavati ; Mehta, Saroj ; Vaiphei, Kim ; Goel, Ramesh Chand ; Mehta, Satish Kumar. / Study of oxidative-stress in rifampicin-induced hepatic injury in growing rats with and without protein-energy malnutrition. In: Human and Experimental Toxicology. 1997 ; Vol. 16, No. 6. pp. 315-321.
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