Study of neurovascular coupling functions for transient focal cerebral ischemia in rats using electrocorticography functional photoacoustic microscopy (ECoG-fPAM)

Lun De Liao, Meng Lin Li, Hsin Yi Lai, You Yin Chen, Nitish V. Thakor

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Recently, the functional photoacoustic microscopy (fPAM) system has been proven to be a reliable imaging technique for measuring the total hemoglobin concentration (HbT), cerebral blood volume (CBV) and hemoglobin oxygen saturation (SO2) in single cerebral blood vessels of rats. In this study, we report for the first time the combination of electrocorticography (ECoG) recordings and fPAM (ECoG-fPAM) to investigate functional hemodynamic changes and neuro-vascular coupling in single cortical arterioles of rats with electrical forepaw stimulation after photothrombotic stroke. Because of the optical focusing nature of our fPAM system, photo-induced ischemic stroke targeting on single cortical arterioles can be easily conducted with simple adaptation. Functional cerebral HbT, CBV and SO2 changes associated with the induced stroke in selected arterioles from the anterior cerebral artery system were imaged with a 36 × 65-μm spatial resolution. The ECoG-fPAM system complements existing imaging techniques and has the potential to offer a favorable tool for explicitly studying cerebral hemodynamics and neuro-vascular coupling in small animal models of photo-induced ischemic stroke.

Original languageEnglish (US)
Title of host publication2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
Pages1799-1802
Number of pages4
DOIs
StatePublished - Oct 31 2013
Event2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 - Osaka, Japan
Duration: Jul 3 2013Jul 7 2013

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)1557-170X

Other

Other2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
CountryJapan
CityOsaka
Period7/3/137/7/13

Keywords

  • Cerebral hemodynamic response
  • Electrocorticography (ECoG)
  • Forepaw electrical stimulation
  • Functional photoacoustic microscopy
  • Photothrombotic stroke
  • Somatosensory-evoked potentials (SSEPs)

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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