TY - JOUR
T1 - Study of multiple human papillomavirus-related lesions of the lower female genital tract by in situ hybridization
AU - Pilotti, Silvana
AU - Gupta, Jean
AU - Stefanon, Bernardina
AU - De Palo, Giuseppe
AU - Shah, Keerti V.
AU - Rilke, Franco
N1 - Funding Information:
From the Division of Anatomical Pathology and Cytology and the Division of Clinical Oncology A, lstituto Nazionale per lo Studio e la Cura dei Tumori, Milan, Italy; and the Department of Immunology and Infectious Diseases, The Johns Hopkins University, School of Hygiene and Public Health. Baltimore. Accepted for publication July 19, 1988. Supported in part by a dedicated research contract from the Ministry of Health, Rome (F.R.) and by National Institutes of Health grant no. POIAI 16959 (K.V.S.). F! words; human papillomavirus. female genital tract, in situ hybrlchzation, intraepithelial neoplasia. Address correspondence and reprint requests to Silvana Pi-lotti, MD, Anatomia Patologica e Citologia, Istituto Nazionale Tu-mori, Via Venezian, 1, 20133 Milano, Italy. 0 1989 by W.B. Saunders Company. 0046-B 177/8912002-0006$5.00/O
PY - 1989/2
Y1 - 1989/2
N2 - Twenty-six women with multiple human papillomavirus (HPV)-related lesions of the lower genital tract were investigated by immunohistochemistry for the internal genus-specific capsid antigen of HPV and by DNA-DNA in situ hybridization with 35S-radiolabeled probes for sequences of HPV- 6 11, HPV-16, and HPV-18. The vulva was the most frequently affected site in all of these cases; the cervix was the second most frequently affected site. The lesions displayed the features of papillomavirus infection in 14 patients, and there was also histologic evidence of early neoplasia in 12 patients. The mean age of patients with and without neoplasia was 40 and 30 years, respectively. Evidence of HPV association was found in 73% of the vulvar lesions and in 40% of the other synchronous lesions by one or both methods. Viral DNA was found in 67% of patients with neoplasia and in 71% of patients without neoplasia. Eleven of 12 HPV-positive patients with neoplasia revealed the presence of HPV-16 in their tissues by in situ hybridization. On the other hand, 50% of those without neoplasia had HPV-16 DNA, whereas the presence of HPV- 6 11 was found in the other 50%. The clinical course of the disease, the distribution of HPV type, and the type of antigen in patients with and without neoplasia suggest that progression to neoplasia was associated with HPV-16. These results stress the practical value of the in situ hybridization method for the identification of those patients with HPV infection who are at risk for progression to malignancy.
AB - Twenty-six women with multiple human papillomavirus (HPV)-related lesions of the lower genital tract were investigated by immunohistochemistry for the internal genus-specific capsid antigen of HPV and by DNA-DNA in situ hybridization with 35S-radiolabeled probes for sequences of HPV- 6 11, HPV-16, and HPV-18. The vulva was the most frequently affected site in all of these cases; the cervix was the second most frequently affected site. The lesions displayed the features of papillomavirus infection in 14 patients, and there was also histologic evidence of early neoplasia in 12 patients. The mean age of patients with and without neoplasia was 40 and 30 years, respectively. Evidence of HPV association was found in 73% of the vulvar lesions and in 40% of the other synchronous lesions by one or both methods. Viral DNA was found in 67% of patients with neoplasia and in 71% of patients without neoplasia. Eleven of 12 HPV-positive patients with neoplasia revealed the presence of HPV-16 in their tissues by in situ hybridization. On the other hand, 50% of those without neoplasia had HPV-16 DNA, whereas the presence of HPV- 6 11 was found in the other 50%. The clinical course of the disease, the distribution of HPV type, and the type of antigen in patients with and without neoplasia suggest that progression to neoplasia was associated with HPV-16. These results stress the practical value of the in situ hybridization method for the identification of those patients with HPV infection who are at risk for progression to malignancy.
KW - female genital tract
KW - human papillomavirus
KW - in situ hybridization
KW - intraepithelial neoplasia
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U2 - 10.1016/0046-8177(89)90175-5
DO - 10.1016/0046-8177(89)90175-5
M3 - Article
C2 - 2536630
AN - SCOPUS:0024581186
SN - 0046-8177
VL - 20
SP - 118
EP - 123
JO - Human pathology
JF - Human pathology
IS - 2
ER -