Study of alginate/chitosan microcapsules for immobilization of Escherichia coli DH5 alpha

Ying Li Fu, Ying Xiong, Xiu Dong Liu, Wei Ting Yu, Yi Li Wang, Xing Ju Yu, Xiao Jun Ma

Research output: Contribution to journalArticlepeer-review

Abstract

Objective proteins synthesized from genetically recombined Escherichia coli strain (E. coli) have been successfully produced by microbe fermentation, but complicated separation and purification steps always make against the maintenance of activities as well as increase the cost. Aiming at simplifying the process, an idea of administrating directly the microencapsulated genetically recombined E. coli is proposed. In this paper, study on culture of E. coli DH5 alpha immobilized in alginate/chitosan (ACA) microcapsule is presented. It was found that E. coli DH5 alpha grew well in the microcapsule with stable growth period longer than that of suspension culture, and cell aggregation phenomenon was observed. In vivo experiments showed that ACA microcapsules with E. coli DH5 alpha stayed over 48 h in mouse intestine, and the morphology of microcapsules was kept intact. These preliminary results have demonstrated that administration of microencapsulated E. coli DH5 alpha is safe, which laied the foundation for microencapsulated genetically recombined E. coli as carriers of gene engineering drugs.

Original languageEnglish (US)
Pages (from-to)239-241
Number of pages3
JournalSheng wu gong cheng xue bao = Chinese journal of biotechnology
Volume18
Issue number2
StatePublished - Jan 2002
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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