STUDIES WITH MURINE LPC‐1 PLASMACYTOMA USING [6‐14C]ARGININE

R. L. Humphrey, S. M. Trivedi, Carmelita G. Frondoza

Research output: Contribution to journalArticle

Abstract

[6‐14C]Arginine ([6‐14C]Arg) was used as an in vivo pulse label to study BALB/c murine LPC‐1 plasmacytoma synthesis and secretion of its tumour‐associated M component (IgG2a, k). With this isotope, an eight‐ to ten‐fold enhancement in the labelling of the γ globulin region and ten‐fold reduction in the albumin labelling were observed. Production and secretion of the M component was detected (within 30 min) after cell transfer. Only mice which received tumour cells showed significant labelling in the γ globulin region 24 hr after isotope injection. The labelling behaviour of the tumour M component correlated with the administered cell dose. The peak heights of radioactivity in the γ region increased with increments in cell number. When the percentage radioactivity diverted into M component was plotted as a function of cell dose, a linear relationship was noted. This study demonstrates the feasibility of using [6‐14C]Arg as a tool to follow the newly synthesized tumour‐associated protein, and provides a means of estimating tumour cell number.

Original languageEnglish (US)
Pages (from-to)71-80
Number of pages10
JournalCell Proliferation
Volume12
Issue number1
DOIs
StatePublished - Jan 1979

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'STUDIES WITH MURINE LPC‐1 PLASMACYTOMA USING [6‐<sup>14</sup>C]ARGININE'. Together they form a unique fingerprint.

  • Cite this