Studies with 1,2-dithiole-3-thione as a chemoprotector of hydroquinone-induced toxicity to DBA/2-derived bone marrow stromal cells

L. E. Twerdok, S. J. Rembish, M. A. Trush

Research output: Contribution to journalArticle

Abstract

Stromal cells from DBA/2 mouse bone marrow have been shown to be susceptible to cytotoxicity induced by several redox-active metabolites of benzene, including hydroquinone (HQ). Treatment with HQ also alters the compositition of stromal cell population by preferentially killing stromal macrophages compared to stromal fibroblasts. This cytotoxicity can be prevented by 1,2-dithiole-3-thione (DTT) as a result of the induction of quinone reductase (QR), a quinone-processing enzyme and glutathione. The inductive activities of DTT protected stromal cells against HQ-induced impairment of stromal cell ability to support myelopoiesis. In vivo feeding of DTT to DBA/2 mice increased QR activity within the bone marrow compartment and protected bone marrow stomal cells isolated from the DTT fed animals from ex vivo HQ challenge. Thus, the inducibility of cellular defense mechanisims and xenobiotic-processing enzymes by chemoprotective agents such as DTT may be a useful stategy for protecting against chemically induced bone marrow toxicities.

Original languageEnglish (US)
Pages (from-to)172-177
Number of pages6
JournalEnvironmental Health Perspectives
Volume101
Issue number2
Publication statusPublished - 1993
Externally publishedYes

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Keywords

  • Bone marrow
  • Chemoprotection
  • DBA/2
  • Hydoquinone
  • Quinone reductase
  • Stromal cells

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

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