Studies using immobilized platelet glycoproteins for detection of platelet alloantibodies

H. O. Kim, S. D. Kennedy, T. S. Kickler

Research output: Contribution to journalArticle

Abstract

The utility of an enzyme linked immunoassay for the detection of platelet alloantibodies to isolated glycoproteins employing commercially available monoclonal antibodies was studied. The authors established the usefulness of the assay for antibodies to platelet alloantigens (APAA) by testing its sensitivity and specificity under different conditions and using a variety of alloantibodies from patients with immune mediated thrombocytopenia. The authors were able to specifically detect a variety of antibodies to platelet alloantigens on different platelet glycoproteins. These antibodies had specificity for HPA-1a (Pl(A1)), HPA-3 (Bak) and HPA-5 (Br). These results show that the APAA is easily adaptable for routine diagnostic testing in hospital laboratories using commercially available monoclonal antibodies and for the diagnosis of alloimmune mediated thrombocytopenic conditions.

Original languageEnglish (US)
Pages (from-to)258-263
Number of pages6
JournalAmerican journal of clinical pathology
Volume104
Issue number3
DOIs
StatePublished - Jan 1 1995

Keywords

  • Human platelet antigens
  • Platelet alloantibody
  • Platelet antibody assay

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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