Studies on the rate and site-specificity of P element transposition

C. A. Berg, A. C. Spradling

Research output: Contribution to journalArticle

Abstract

A single genetically marked P element can be efficiently mobilized to insertionally mutagenize the Drosophila genome. We have investigated how the structure of the starting element and its location along the X chromosome influenced the rate and location of mutations recovered. The structure of two P[rosy+] elements strongly affected mobilization by the autonomous 'Jumpstarter-1' element. Their average transposition rates differed more than 12-fold, while their initial chromosomal location had a smaller effect. The lethal and sterile mutations induced by mobilizing a P[rosy+] element from position 1F were compared with those identified previously using a P[neo(R)] element at position 9C. With one possible exception, insertion hotspots for one element were frequently also targets of the other transposon. These experiments suggested that the genomic location of a P element does not usually influence its target sites on nonhomologous chromosomes. During the course of these experiments, Y-linked insertions expressing rosy+ were recovered, suggesting that marked P elements can sometimes insert and function at heterochromatic sites.

Original languageEnglish (US)
Pages (from-to)515-524
Number of pages10
JournalGenetics
Volume127
Issue number3
StatePublished - 1991
Externally publishedYes

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X Chromosome
Mutation Rate
Drosophila
Chromosomes
Genome
Mutation

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Berg, C. A., & Spradling, A. C. (1991). Studies on the rate and site-specificity of P element transposition. Genetics, 127(3), 515-524.

Studies on the rate and site-specificity of P element transposition. / Berg, C. A.; Spradling, A. C.

In: Genetics, Vol. 127, No. 3, 1991, p. 515-524.

Research output: Contribution to journalArticle

Berg, CA & Spradling, AC 1991, 'Studies on the rate and site-specificity of P element transposition', Genetics, vol. 127, no. 3, pp. 515-524.
Berg, C. A. ; Spradling, A. C. / Studies on the rate and site-specificity of P element transposition. In: Genetics. 1991 ; Vol. 127, No. 3. pp. 515-524.
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