These studies were addressed to the hypothesis that interactions between a target cell's plasma membrane and its nucleus might be of significance in determining the susceptibility of the cell to lysis by cytotoxic lymphocytes. To this end, P815 mastocytoma cells were enucleated by centrifugation through a discontinuous Ficoll gradient containing 20 μg/ml cytochalasin B. Two types of vesicles were isolated, both enclosed by plasma membrane: one fraction, termed cytoplasts, contained significant quantities of the cytoplasmic marker lactate dehydrogenase, but lacked nuclei by both biochemical and phase-contrast microscopic criteria. The other type, karyoplasts, contained nuclei. Cytoplasts displayed surface H-2 antigens but 'capped' these molecules poorly; karyoplasts showed a 'capping' capacity similar to that of intact cells. Despite a deficient capacity to 'cap' surface H-2, cytoplasts were susceptible to the action of cytotoxic T cells. There was no indication that cytoplasts were more resistant to lysis than either karyoplasts or intact cells. Moreover, sources of NK, K, and lectin-dependent cytotoxic cells were all capable of including specific 51Cr release from both cytoplast and karyoplast preparations. Thus, in keeping with our earlier suggestion that the target cell plays a passive role in the lytic process, these studies show that possession of a nucleus is not a prerequisite for susceptibility to cell-mediated cytoxicity.
|Original language||English (US)|
|Number of pages||6|
|Journal||Journal of Immunology|
|State||Published - Dec 1 1978|
ASJC Scopus subject areas
- Immunology and Allergy