Studies on the mechanism of inhibition of 2-acetylaminofluorene toxicity by butylated hydroxytoluene

Jay I. Goodman, James E. Trosko, James D Yager

Research output: Contribution to journalArticle

Abstract

Male rats were placed on a diet containing 0.05% (w/w) of the hepatic carcinogen 2-acetylaminofluorene (AAF). They ceased to gain weight. However, when the carcinogenic diet was supplemented with butylated hydroxytoluene (BHT) (0.5% w/w), an antioxidant, the animals gained weight at approximately one-half the normal rate. This observation led to a series of experiments aimed at elucidating the mechanism(s) by which BHT reduced the toxicity of AAF. These initial studies were directed towards the effect of BHT on the extent and duration of the covalent binding of AAF with DNA. BHT feeding was shown to reduce the binding of carcinogen to hepatic DNA. Studies employing cells in culture demonstrated that BHT does not influence either excision repair or post-replication repair of DNA. These data indicate that a potential mechanism of action of BHT is at the anti-initiation level of carcinogen-induced DNA damage.

Original languageEnglish (US)
Pages (from-to)171-182
Number of pages12
JournalChemico-Biological Interactions
Volume12
Issue number2
DOIs
StatePublished - 1976
Externally publishedYes

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ASJC Scopus subject areas

  • Toxicology

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