Studies on the biochemical basis of spontaneous mutation. II. The incorporation of a base and its analogue into DNA by wild-type, mutator and antimutator DNA polymerases

Maurice J. Bessman, Nicholas Muzyczka, Myron F. Goodman, Ronald L. Schnaar

Research output: Contribution to journalArticlepeer-review

Abstract

The incorporation and turnover of adenine and its analogue 2-aminopurine into DNA by purified wild-type, mutator, and antimutator T4 DNA polymerase have been measured. Antimutators incorporate less 2-aminopurine into DNA than does wild type, and imitators incorporate more than wild type. Analysis of these data is consistent with the idea that the incorporation of 2-aminopurine is influenced primarily by the ratio of 3′-exonuclease to polymerase activities of the different enzymes. The experimental results conform to a model equation, which expresses the base incorporation frequency as a function of the polymerase insertion and removal activities. Some of the implications of the model equation are examined in this and the accompanying paper.

Original languageEnglish (US)
Pages (from-to)409-421
Number of pages13
JournalJournal of molecular biology
Volume88
Issue number2
DOIs
StatePublished - Sep 15 1974

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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