Studies on acetylated human hemoglobin

E. Bucci, Clara Fronticelli, L. Bellelli, E. Antonini, J. Wyman, A. Rossi Fanelli

Research output: Contribution to journalArticle

Abstract

Preparations of human hemoglobin of various degree of acetylation have been made. The compounds are stable and are spectroscopically indistinguishable from normal hemoglobin in the visible region. The molecular weights appear to be reduced to about half that of normal hemoglobin at the higher degrees of acetylation. The isoelectric points are shifted downward to pH 3.5 - 5.0, depending on the degree of acetylation. Even at the highest degree of acetylation achieved (~80%), the protein still combines reversibly with oxygen. However the value of the coefficient n of the Hill equation is reduced below its normal value and is no longer independent of pH; also, the value of log p 1 2 is always lower than that for normal hemoglobin and tends to be independent of pH; i.e., the Bohr effect is largely eliminated. The number of sulfhydryl groups titratable with p-mercuribenzoate is reduced to zero.

Original languageEnglish (US)
Pages (from-to)364-368
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume100
Issue number3
StatePublished - Mar 1963
Externally publishedYes

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Acetylation
Hemoglobins
Isoelectric Point
Reference Values
Molecular Weight
Molecular weight
Oxygen
acetylated hemoglobin
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Bucci, E., Fronticelli, C., Bellelli, L., Antonini, E., Wyman, J., & Fanelli, A. R. (1963). Studies on acetylated human hemoglobin. Archives of Biochemistry and Biophysics, 100(3), 364-368.

Studies on acetylated human hemoglobin. / Bucci, E.; Fronticelli, Clara; Bellelli, L.; Antonini, E.; Wyman, J.; Fanelli, A. Rossi.

In: Archives of Biochemistry and Biophysics, Vol. 100, No. 3, 03.1963, p. 364-368.

Research output: Contribution to journalArticle

Bucci, E, Fronticelli, C, Bellelli, L, Antonini, E, Wyman, J & Fanelli, AR 1963, 'Studies on acetylated human hemoglobin', Archives of Biochemistry and Biophysics, vol. 100, no. 3, pp. 364-368.
Bucci E, Fronticelli C, Bellelli L, Antonini E, Wyman J, Fanelli AR. Studies on acetylated human hemoglobin. Archives of Biochemistry and Biophysics. 1963 Mar;100(3):364-368.
Bucci, E. ; Fronticelli, Clara ; Bellelli, L. ; Antonini, E. ; Wyman, J. ; Fanelli, A. Rossi. / Studies on acetylated human hemoglobin. In: Archives of Biochemistry and Biophysics. 1963 ; Vol. 100, No. 3. pp. 364-368.
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