Studies of X chromosome DNA methylation in normal human cells

Stanley F. Wolf, Barbara R Migeon

Research output: Contribution to journalArticle

Abstract

Studies of methylation along 28 kilobases of X-chromosome DNA, assayed by Southern blot analysis using cloned X-chromosome-specific probes, indicates that XDNA methylation in normal human cells changes with replication, is not correlated with number of X chromosomes or transcriptional activity, and is less stable and more prevalent than when the human X is in the foreign environment of mouse-human hybrid cells. In contrast with observations of others in heteroploid cells, we observed no derepression of the inactive X in clonal populations of normal human fibroblasts treated with 5-azacytidine. This may reflect the differences in stability of the methylation of the human X in a foreign environment. Our observations preclude ubiquitous methylation differences as the molecular basis for X-chromosome inactivation.

Original languageEnglish (US)
Pages (from-to)667-671
Number of pages5
JournalNature
Volume295
Issue number5851
DOIs
StatePublished - 1982

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X Chromosome
DNA Methylation
Methylation
Azacitidine
X Chromosome Inactivation
Hybrid Cells
Southern Blotting
Fibroblasts
DNA
Population

ASJC Scopus subject areas

  • General

Cite this

Studies of X chromosome DNA methylation in normal human cells. / Wolf, Stanley F.; Migeon, Barbara R.

In: Nature, Vol. 295, No. 5851, 1982, p. 667-671.

Research output: Contribution to journalArticle

Wolf, Stanley F. ; Migeon, Barbara R. / Studies of X chromosome DNA methylation in normal human cells. In: Nature. 1982 ; Vol. 295, No. 5851. pp. 667-671.
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