Studies of Simian virus 40 DNA. VII. A cleavage map of the SV40 genome

Kathleen J. Danna, George Henry Sack, Daniel Nathans

Research output: Contribution to journalArticle

Abstract

A physical map of the Simian virus 40 genome has been constructed on the basis of specific cleavage of Simian virus 40 DNA by bacterial restriction endonucleases. The 11 fragments produced by enzyme from Hemophilus influenzae have been ordered by analysis of partial digest products and by analysis of an overlapping set of fragments produced by enzyme from Hemophilus parainfluenzae. In addition, the single site in SV40 DNA cleaved by the Escherichia coli RI restriction endonuclease has been located. With this site as a reference point, the H. influenzae cleavage sites and the H. parainfluenzae cleavage sites have been localized on the map.

Original languageEnglish (US)
JournalJournal of Molecular Biology
Volume78
Issue number2
DOIs
StatePublished - Aug 5 1973

Fingerprint

Haemophilus parainfluenzae
Simian virus 40
DNA Restriction Enzymes
Haemophilus influenzae
Genome
Bacterial DNA
DNA
Enzymes
Escherichia coli

ASJC Scopus subject areas

  • Virology

Cite this

Studies of Simian virus 40 DNA. VII. A cleavage map of the SV40 genome. / Danna, Kathleen J.; Sack, George Henry; Nathans, Daniel.

In: Journal of Molecular Biology, Vol. 78, No. 2, 05.08.1973.

Research output: Contribution to journalArticle

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