TY - JOUR
T1 - Studies of serum-free culture medium in the generation of lymphokine activated killer cells
AU - Mesler Muul, Linda
AU - Nason-Burchenal, Kathryn
AU - Hyatt, Cornelia
AU - Schwarz, Susan
AU - Slavin, Dale
AU - Director, Elaine P.
AU - Rosenberg, Steven A.
PY - 1987/12/24
Y1 - 1987/12/24
N2 - Lymphokine activated killer (LAK) cells administered in conjunction with recombinant interleukin-2 can mediate the regression of metastatic tumor in some patients with advanced cancer. In these trials LAK cells were activated in medium containing 2% human type A or AB serum. We have found three commercially available, serum-free culture media which allow development of in vitro LAK activity by human peripheral blood lymphocytes. They are AIMV (Gibco), MASF-3 (Whitaker-MA Bioproducts) and HB-104 (Dupont). If 2-mercaptoethanol was added to these culture media they were also capable of generating murine LAK cells which were effective in reducing pulmonary metastases in the murine MCA-106 model. Although LAK cells generated in these media have not been tested in humans yet, potentially they could provide a safe, unlimited and less expensive source of culture fluid for generating the large numbers of LAK cells needed for human clinical trials.
AB - Lymphokine activated killer (LAK) cells administered in conjunction with recombinant interleukin-2 can mediate the regression of metastatic tumor in some patients with advanced cancer. In these trials LAK cells were activated in medium containing 2% human type A or AB serum. We have found three commercially available, serum-free culture media which allow development of in vitro LAK activity by human peripheral blood lymphocytes. They are AIMV (Gibco), MASF-3 (Whitaker-MA Bioproducts) and HB-104 (Dupont). If 2-mercaptoethanol was added to these culture media they were also capable of generating murine LAK cells which were effective in reducing pulmonary metastases in the murine MCA-106 model. Although LAK cells generated in these media have not been tested in humans yet, potentially they could provide a safe, unlimited and less expensive source of culture fluid for generating the large numbers of LAK cells needed for human clinical trials.
KW - Immunotherapy, adoptive
KW - Interleukin-2, recombinant
KW - Lymphokine-activated killer cell
KW - Serum-free culture medium
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U2 - 10.1016/0022-1759(87)90265-1
DO - 10.1016/0022-1759(87)90265-1
M3 - Article
C2 - 3693906
AN - SCOPUS:0023572106
SN - 0022-1759
VL - 105
SP - 183
EP - 192
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 2
ER -