Studies concerning the structure and organization of the vaccinia virus nucleoid. I. Isolation and characterization of subviral particles prepared by treating virions with guanidine-HCI, nonidet-P40, and 2-mercaptoethanol

M. J. Soloski, C. V. Cabrera, M. Estevan, J. A. Holowczak

Research output: Contribution to journalArticlepeer-review

Abstract

Subviral particles (Gu-subviral particles) containing DNA complexed with 20-25% of the protein found in complete virus particles could be isolated after treating purified vaccinia virions with 8 M guanidine-HCI, 1% Nonidet-P40, and 1% 2-mercaptoethanol. Electron microscopic examination of the Gu-subviral particles fixed with glutaraldehyde and formaldehyde, revealed spherical structures, 300-350 nm in diameter. DNA in combination with protein was observed to be further folded into globular structures, 200-600 Å in diameter on the surface of the particles. Analysis of the proteins in Gu-subviral particles by SDS-polyacrylamide gel electrophoresis showed that 10 polypeptides with molecular weights of 120K, 90K, 68K, 61K, 58K, 32K, 27K, 24K, 14.5K, and 10K were associated with the viral DNA. After dialysis, the particles could be shown to have retained, in part, the capacity to catalyze the synthesis of RNA in vitro.

Original languageEnglish (US)
Pages (from-to)209-217
Number of pages9
JournalVirology
Volume99
Issue number2
DOIs
StatePublished - Dec 1979
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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