Strychnine binding associated with synaptic glycine receptors in rat spinal cord membranes: Ionic influences

Walter E. Muller, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Ammonium salts of some anions decrease the potency of glycine in inhibiting [3H]strychnine binding associated with synaptic glycine receptors. A correspondence exists between the ability of the ammonium salts of anions to increase the IC50 of glycine in inhibiting the [3H]strychnine binding, their capacity to reduce the [3H] strychnine binding itself, and their capacity to reverse inhibitory postsynaptic potentials. The decrease of [3H]strychnine binding in the presence of chloride is abolished by sodium, while the decrease of the potency of glycine in inhibiting [3H]strychnine is not. Binding of [3H]strychnine is influenced by monovalent cations in a biphasic fashion. Concentrations of Li+, K+, and Na+ up to 150 mM decrease [3H]strychnine binding, while higher concentrations of the cations increase [3H]strychnine binding. Inhibition by glycine of [3H]strychnine binding is enhanced by low concentrations of these cations.

Original languageEnglish (US)
Pages (from-to)107-116
Number of pages10
JournalBrain Research
Volume147
Issue number1
DOIs
StatePublished - May 19 1978

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Glycine Receptors
Strychnine
Neurotransmitter Receptor
Spinal Cord
Membranes
Glycine
Ammonium Compounds
Anions
Cations
Salts
Monovalent Cations
Inhibitory Postsynaptic Potentials
Inhibitory Concentration 50
Chlorides
Sodium

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Strychnine binding associated with synaptic glycine receptors in rat spinal cord membranes : Ionic influences. / Muller, Walter E.; Snyder, Solomon H.

In: Brain Research, Vol. 147, No. 1, 19.05.1978, p. 107-116.

Research output: Contribution to journalArticle

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