Strychnine binding associated with synaptic glycine receptors in rat spinal cord membranes: Ionic influences

Walter E. Muller, Solomon H. Snyder

Research output: Contribution to journalArticlepeer-review

Abstract

Ammonium salts of some anions decrease the potency of glycine in inhibiting [3H]strychnine binding associated with synaptic glycine receptors. A correspondence exists between the ability of the ammonium salts of anions to increase the IC50 of glycine in inhibiting the [3H]strychnine binding, their capacity to reduce the [3H] strychnine binding itself, and their capacity to reverse inhibitory postsynaptic potentials. The decrease of [3H]strychnine binding in the presence of chloride is abolished by sodium, while the decrease of the potency of glycine in inhibiting [3H]strychnine is not. Binding of [3H]strychnine is influenced by monovalent cations in a biphasic fashion. Concentrations of Li+, K+, and Na+ up to 150 mM decrease [3H]strychnine binding, while higher concentrations of the cations increase [3H]strychnine binding. Inhibition by glycine of [3H]strychnine binding is enhanced by low concentrations of these cations.

Original languageEnglish (US)
Pages (from-to)107-116
Number of pages10
JournalBrain research
Volume147
Issue number1
DOIs
StatePublished - May 19 1978

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

Fingerprint Dive into the research topics of 'Strychnine binding associated with synaptic glycine receptors in rat spinal cord membranes: Ionic influences'. Together they form a unique fingerprint.

Cite this