Structure–function relationship of the M2 ion channel of influenza A virus

Jorgen A. Mould, Kevin Shuck, Jason E. Drury, Stephan M. Frings, U. Benjamin Kaupp, Andrew Pekosz, Robert A. Lamb, Lawrence H. Pinto

Research output: Contribution to journalArticle

Abstract

The use of oligomers comprised of amantadine-sensitive and -resistant forms demonstrated that the active oligomeric state of the channel is a tetramer. Cysteine scanning mutagenesis followed by evaluation of the ability of sulfhydryl-specific reagents to inhibit the channel demonstrated that each monomer is a coiled coil and that the pore-lining residues are Val-27, Ala-30, Gly-34, His-37 and Trp-41. Under oxidizing conditions, mutant proteins containing cysteine at or close to these residues form dimers, but do so less readily at low pH for residues near #41, supporting the notion that a pH-induced conformational change occurs in this region of the protein. A functional change in state was detected by comparing the efflux of H+ from acid-loaded cells expressing M2 protein with those treated with the protonophore FCCP: the efflux was high for FCCP-treated cells, but not M2-expressing cells when pHout was elevated. The current of the wild-type protein is carried by H+ and is increased by low pHout, but replacement of the His-37 results in pH-independent currents of other ions as well, suggesting that the selectivity and activation of the channel might result from the action of His-37. The C-terminus is needed for sustained function of the channel, as truncation mutants are abnormal and that they pass H+ for only a short time.

Original languageEnglish (US)
Pages (from-to)389-396
Number of pages8
JournalInternational Congress Series
Volume1219
Issue numberC
DOIs
StatePublished - Oct 1 2001
Externally publishedYes

Keywords

  • Amantadine
  • Ion channels

ASJC Scopus subject areas

  • Medicine(all)

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  • Cite this

    Mould, J. A., Shuck, K., Drury, J. E., Frings, S. M., Kaupp, U. B., Pekosz, A., Lamb, R. A., & Pinto, L. H. (2001). Structure–function relationship of the M2 ion channel of influenza A virus. International Congress Series, 1219(C), 389-396. https://doi.org/10.1016/S0531-5131(01)00641-0