Structure of the RECK CC domain, an evolutionary anomaly

Tao Hsin Chang, Fu Lien Hsieh, Philip M. Smallwood, Sandra B. Gabelli, Jeremy Nathans

Research output: Contribution to journalArticlepeer-review

Abstract

Five small protein domains, the CC-domains, at the N terminus of the RECK protein, play essential roles in signaling by WNT7A and WNT7B in the context of central nervous system angiogenesis and blood-brain barrier formation and maintenance. We have determined the structure of CC domain 4 (CC4) at 1.65-A resolution and find that it folds into a compact four-helix bundle with three disulfide bonds. The CC4 structure, together with homology modeling of CC1, reveals the surface locations of critical residues that were shown in previous mutagenesis studies to mediate GPR124 binding and WNT7A/WNT7B recognition and signaling. Surprisingly, sequence and structural homology searches reveal no other cellsurface or secreted domains in vertebrates that resemble the CC domain, a pattern that is in striking contrast to other ancient and similarly sized domains, such as Epidermal Growth Factor, Fibronectin Type 3, Immunoglobulin, and Thrombospondin type 1 domains, which are collectively present in hundreds of proteins.

Original languageEnglish (US)
Pages (from-to)15104-15111
Number of pages8
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number26
DOIs
StatePublished - Jun 30 2020

Keywords

  • Blood-brain barrier
  • Extracellular domain
  • Four-helix bundle
  • Protein evolution
  • Wnt signaling

ASJC Scopus subject areas

  • General

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