Structure of the novel heme adduct formed during the reaction of human hemoglobin with BrCCl3 in red cell lysates

Yoichi Osawa, Christine S. Fellows, Carl A. Meyer, Amina Woods, John A. Castoro, Robert J. Cotter, Charles L. Wilkins, Robert J. Highet

Research output: Contribution to journalArticle

Abstract

It was previously shown that the reductive debromination of BrCCl3 to trichloromethyl radical by human hemoglobin leads to formation of dissociable altered heme products, two of which are identical to those formed from myoglobin and one which is novel. In this study, we have elucidated the structure of this novel adduct with the use of mass spectrometry, as well as 1H and 13C NMR as a substitution product of a -C(Cl)=CCl2 moiety for a β-hydrogen atom on the prosthetic heme's ring I vinyl group. From studies with the use of 13C-enriched BrCCl3, it was determined that the added carbon atoms were derived from 2 eq of BrCCl3. A mechanism that involves multiple reductive events and a radical cation heme intermediate is proposed. Consistent with this mechanism, cellular reductants were found to selectively enhance the amount of this novel dissociable heme adduct. These studies reveal fine differences between myoglobin and hemoglobin in the accessibility of reactive intermediates to the ring I vinyl group, as well as the potential importance of cellular reductants on the course of heme alteration.

Original languageEnglish (US)
Pages (from-to)15481-15487
Number of pages7
JournalJournal of Biological Chemistry
Volume269
Issue number22
StatePublished - Jun 3 1994

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Heme
Hemoglobins
Cells
Myoglobin
Reducing Agents
Atoms
Prosthetics
Mass spectrometry
Cations
Hydrogen
Mass Spectrometry
Substitution reactions
Carbon
Nuclear magnetic resonance

ASJC Scopus subject areas

  • Biochemistry

Cite this

Osawa, Y., Fellows, C. S., Meyer, C. A., Woods, A., Castoro, J. A., Cotter, R. J., ... Highet, R. J. (1994). Structure of the novel heme adduct formed during the reaction of human hemoglobin with BrCCl3 in red cell lysates. Journal of Biological Chemistry, 269(22), 15481-15487.

Structure of the novel heme adduct formed during the reaction of human hemoglobin with BrCCl3 in red cell lysates. / Osawa, Yoichi; Fellows, Christine S.; Meyer, Carl A.; Woods, Amina; Castoro, John A.; Cotter, Robert J.; Wilkins, Charles L.; Highet, Robert J.

In: Journal of Biological Chemistry, Vol. 269, No. 22, 03.06.1994, p. 15481-15487.

Research output: Contribution to journalArticle

Osawa, Y, Fellows, CS, Meyer, CA, Woods, A, Castoro, JA, Cotter, RJ, Wilkins, CL & Highet, RJ 1994, 'Structure of the novel heme adduct formed during the reaction of human hemoglobin with BrCCl3 in red cell lysates', Journal of Biological Chemistry, vol. 269, no. 22, pp. 15481-15487.
Osawa, Yoichi ; Fellows, Christine S. ; Meyer, Carl A. ; Woods, Amina ; Castoro, John A. ; Cotter, Robert J. ; Wilkins, Charles L. ; Highet, Robert J. / Structure of the novel heme adduct formed during the reaction of human hemoglobin with BrCCl3 in red cell lysates. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 22. pp. 15481-15487.
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