TY - JOUR
T1 - Structure of the mammalian kinin receptor gene locus
AU - Cayla, C. écile
AU - Merino, Vanessa Ferreira
AU - Cabrini, Daniela Almeida
AU - Silva, José Antonio
AU - Pesquero, João Bosco
AU - Bader, Michael
N1 - Funding Information:
This work was supported by a grant (PROBRAL) from CAPES and DAAD and by a joined grant of the Ministeries of Science in Brazil and Germany. Several authors were supported by fellowships of different institutions: C.C. by the MDC, V.F.M. and J.A.S. Jr by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP), D.A.C. by Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq).
PY - 2002/12
Y1 - 2002/12
N2 - The genes encoding the two kinin receptors, B1 and B2, are closely linked on the same chromosome in human, mouse, and rat. In this article, we present the organisation of the B1B2 locus in these mammals. This organisation was obtained by comparing the kinin receptor mRNA sequences of man and mouse with the sequence of chromosomes 14 and 12, respectively. We found that the two genes are located in tandem orientation, separated by only 7.8 kb in mice and 12 kb in humans. The distance of the two genes on rat chromosome 6 was determined by long-range PCR to be 9.5 kb. The organisation of the two genes encoding the kinin receptors is similar in the three species, except that the human B1 gene harbors an additional exon, which may originate from the insertion of an Alu repetitive sequence during evolution. Moreover, the human and rat, but not the murine, B2 genes carry an alternatively spliced exon between exons 2 and 3, termed exon 2b.
AB - The genes encoding the two kinin receptors, B1 and B2, are closely linked on the same chromosome in human, mouse, and rat. In this article, we present the organisation of the B1B2 locus in these mammals. This organisation was obtained by comparing the kinin receptor mRNA sequences of man and mouse with the sequence of chromosomes 14 and 12, respectively. We found that the two genes are located in tandem orientation, separated by only 7.8 kb in mice and 12 kb in humans. The distance of the two genes on rat chromosome 6 was determined by long-range PCR to be 9.5 kb. The organisation of the two genes encoding the kinin receptors is similar in the three species, except that the human B1 gene harbors an additional exon, which may originate from the insertion of an Alu repetitive sequence during evolution. Moreover, the human and rat, but not the murine, B2 genes carry an alternatively spliced exon between exons 2 and 3, termed exon 2b.
KW - Bradykinin
KW - Gene
KW - Human
KW - Locus
KW - Mouse
KW - Rat
KW - Receptor
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U2 - 10.1016/S1567-5769(02)00175-3
DO - 10.1016/S1567-5769(02)00175-3
M3 - Article
C2 - 12489785
AN - SCOPUS:1842848076
SN - 1567-5769
VL - 2
SP - 1721
EP - 1727
JO - International immunopharmacology
JF - International immunopharmacology
IS - 13-14
ER -