Structure of the human spermidine/spermine N1-acetyltransferase gene. Kxon/intron gene organization and localization to Xp22.1

Lei Xiao, Celano Paul, Amy R. Mank, Constance Griffin, Ethylin Wang Jabs, Anita L. Hawkins, Robert A Casero

Research output: Contribution to journalArticle

Abstract

The super induction of spermidine/spermine N1-acetyltransferase (SSAT), has been implicated in the cytotoxic response of human solid tumors to the bis(ethyl)polyamines. The SSAT response is a phenotype specific response and is modulated at the level of increased steady-state mRNA levels and enzyme protein. The human genomic region (4,095 bases) containing the coding sequence of SSAT has been cloned and localized to the Xp22.1 region. Primer extension analysis indicates the transcription of SSAT starts 179 bases upstream from the translational start site and appears to be under the control of a "TATA-less" promoter. The availability of this human clone will facilitate the direct functional examination of the SSAT gene.

Original languageEnglish (US)
Pages (from-to)1493-1502
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume187
Issue number3
DOIs
StatePublished - Sep 30 1992

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Acetyltransferases
Spermidine
Spermine
Introns
Genes
Polyamines
Transcription
Tumors
Clone Cells
diamine N-acetyltransferase
Availability
Phenotype
Messenger RNA
Enzymes
Neoplasms
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Structure of the human spermidine/spermine N1-acetyltransferase gene. Kxon/intron gene organization and localization to Xp22.1. / Xiao, Lei; Paul, Celano; Mank, Amy R.; Griffin, Constance; Jabs, Ethylin Wang; Hawkins, Anita L.; Casero, Robert A.

In: Biochemical and Biophysical Research Communications, Vol. 187, No. 3, 30.09.1992, p. 1493-1502.

Research output: Contribution to journalArticle

Xiao, Lei ; Paul, Celano ; Mank, Amy R. ; Griffin, Constance ; Jabs, Ethylin Wang ; Hawkins, Anita L. ; Casero, Robert A. / Structure of the human spermidine/spermine N1-acetyltransferase gene. Kxon/intron gene organization and localization to Xp22.1. In: Biochemical and Biophysical Research Communications. 1992 ; Vol. 187, No. 3. pp. 1493-1502.
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