Structure of the human spermidine/spermine N1-acetyltransferase gene. Kxon/intron gene organization and localization to Xp22.1

Lei Xiao; Celano Paul; Amy R. Mank; Constance Griffin; Ethylin Wang Jabs; Anita L. Hawkins; Robert A. Casero

doi:10.1016/0006-291X(92)90471-V

Structure of the human spermidine/spermine N¹-acetyltransferase gene. Kxon/intron gene organization and localization to Xp22.1

Lei Xiao, Celano Paul, Amy R. Mank, Constance Griffin, Ethylin Wang Jabs, Anita L. Hawkins, Robert A. Casero

School of Medicine

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

The super induction of spermidine/spermine N¹-acetyltransferase (SSAT), has been implicated in the cytotoxic response of human solid tumors to the bis(ethyl)polyamines. The SSAT response is a phenotype specific response and is modulated at the level of increased steady-state mRNA levels and enzyme protein. The human genomic region (4,095 bases) containing the coding sequence of SSAT has been cloned and localized to the Xp22.1 region. Primer extension analysis indicates the transcription of SSAT starts 179 bases upstream from the translational start site and appears to be under the control of a "TATA-less" promoter. The availability of this human clone will facilitate the direct functional examination of the SSAT gene.

Original language	English (US)
Pages (from-to)	1493-1502
Number of pages	10
Journal	Biochemical and Biophysical Research Communications
Volume	187
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(92)90471-V
State	Published - Sep 30 1992

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(92)90471-V

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title = "Structure of the human spermidine/spermine N1-acetyltransferase gene. Kxon/intron gene organization and localization to Xp22.1",

abstract = "The super induction of spermidine/spermine N1-acetyltransferase (SSAT), has been implicated in the cytotoxic response of human solid tumors to the bis(ethyl)polyamines. The SSAT response is a phenotype specific response and is modulated at the level of increased steady-state mRNA levels and enzyme protein. The human genomic region (4,095 bases) containing the coding sequence of SSAT has been cloned and localized to the Xp22.1 region. Primer extension analysis indicates the transcription of SSAT starts 179 bases upstream from the translational start site and appears to be under the control of a {"}TATA-less{"} promoter. The availability of this human clone will facilitate the direct functional examination of the SSAT gene.",

author = "Lei Xiao and Celano Paul and Mank, {Amy R.} and Constance Griffin and Jabs, {Ethylin Wang} and Hawkins, {Anita L.} and Casero, {Robert A.}",

note = "Funding Information: A-. The authors vish to thank Drs. Kenneth W. Kinsler and Jon Oliner for helpful discussions, Jeff Smith for expert technical assistance. and Tammy Hess and Sandra Lund for their skilled administrative assistance. This vork was supported in part by NIH Grants CA51085. CA47492. GHlOlS-02, CA06973, and HG00373.",

year = "1992",

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doi = "10.1016/0006-291X(92)90471-V",

language = "English (US)",

volume = "187",

pages = "1493--1502",

journal = "Biochemical and Biophysical Research Communications",

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TY - JOUR

T1 - Structure of the human spermidine/spermine N1-acetyltransferase gene. Kxon/intron gene organization and localization to Xp22.1

AU - Xiao, Lei

AU - Paul, Celano

AU - Mank, Amy R.

AU - Griffin, Constance

AU - Jabs, Ethylin Wang

AU - Hawkins, Anita L.

AU - Casero, Robert A.

N1 - Funding Information: A-. The authors vish to thank Drs. Kenneth W. Kinsler and Jon Oliner for helpful discussions, Jeff Smith for expert technical assistance. and Tammy Hess and Sandra Lund for their skilled administrative assistance. This vork was supported in part by NIH Grants CA51085. CA47492. GHlOlS-02, CA06973, and HG00373.

PY - 1992/9/30

Y1 - 1992/9/30

N2 - The super induction of spermidine/spermine N1-acetyltransferase (SSAT), has been implicated in the cytotoxic response of human solid tumors to the bis(ethyl)polyamines. The SSAT response is a phenotype specific response and is modulated at the level of increased steady-state mRNA levels and enzyme protein. The human genomic region (4,095 bases) containing the coding sequence of SSAT has been cloned and localized to the Xp22.1 region. Primer extension analysis indicates the transcription of SSAT starts 179 bases upstream from the translational start site and appears to be under the control of a "TATA-less" promoter. The availability of this human clone will facilitate the direct functional examination of the SSAT gene.

AB - The super induction of spermidine/spermine N1-acetyltransferase (SSAT), has been implicated in the cytotoxic response of human solid tumors to the bis(ethyl)polyamines. The SSAT response is a phenotype specific response and is modulated at the level of increased steady-state mRNA levels and enzyme protein. The human genomic region (4,095 bases) containing the coding sequence of SSAT has been cloned and localized to the Xp22.1 region. Primer extension analysis indicates the transcription of SSAT starts 179 bases upstream from the translational start site and appears to be under the control of a "TATA-less" promoter. The availability of this human clone will facilitate the direct functional examination of the SSAT gene.

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Structure of the human spermidine/spermine N¹-acetyltransferase gene. Kxon/intron gene organization and localization to Xp22.1

Abstract

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