Structure of the germline genome of Tetrahymena thermophila and relationship to the massively rearranged somatic genome

Eileen P. Hamilton, Aurélie Kapusta, Piroska E. Huvos, Shelby L. Bidwell, Nikhat Zafar, Haibao Tang, Michalis Hadjithomas, Vivek Krishnakumar, Jonathan H. Badger, Elisabet V. Caler, Carsten Russ, Qiandong Zeng, Lin Fan, Joshua Z. Levin, Terrance Shea, Sarah K. Young, Ryan Hegarty, Riza Daza, Sharvari Gujja, Jennifer R. WortmanBruce W. Birren, Chad Nusbaum, Jainy Thomas, Clayton M. Carey, Ellen J. Pritham, Cédric Feschotte, Tomoko Noto, Kazufumi Mochizuki, Romeo Papazyan, Sean D. Taverna, Paul H. Dear, Donna M. Cassidy-Hanley, Jie Xiong, Wei Miao, Eduardo Orias, Robert S. Coyne

Research output: Contribution to journalArticlepeer-review

Abstract

The germline genome of the binucleated ciliate Tetrahymena thermophila undergoes programmed chromosome breakage and massive DNA elimination to generate the somatic genome. Here, we present a complete sequence assembly of the germline genome and analyze multiple features of its structure and its relationship to the somatic genome, shedding light on the mechanisms of genome rearrangement as well as the evolutionary history of this remarkable germline/soma differentiation. Our results strengthen the notion that a complex, dynamic, and ongoing interplay between mobile DNA elements and the host genome have shaped Tetrahymena chromosome structure, locally and globally. Non-standard outcomes of rearrangement events, including the generation of short-lived somatic chromosomes and excision of DNA interrupting protein-coding regions, may represent novel forms of developmental gene regulation. We also compare Tetrahymena’s germline/soma differentiation to that of other characterized ciliates, illustrating the wide diversity of adaptations that have occurred within this phylum.

Original languageEnglish (US)
Article numbere19090
JournaleLife
Volume5
Issue numberNOVEMBER2016
DOIs
StatePublished - Nov 28 2016

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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