Structure of the genome of equine herpesvirus type 1

Berch E. Henry, Robin A. Robinson, Steven A. Dauenhauer, Sally S. Atherton, Gary Selwyn Hayward, Dennis J. O'Callaghan

Research output: Contribution to journalArticle

Abstract

The molecular structure of the genome of equine herpesvirus type 1 (EHV-1) was determined by restriction endonuclease mapping studies. Primary restriction enzyme digestion of purified EHV-1 DNA, either unlabeled, 32P04 labeled, or [3H]TdR labeled, gave the following cleavage patterns: EcoRI yielded 17 fragments of 23.4 to 1.3 megadaltons (Mdalton); BglII, 16 fragments of 24.5 to 1.0 Mdalton; XbaI, 15 major fragments of 18.6 to 1.7 Mdalton; and BamHI,17 fragments of 13.7 to 2.8 Mdalton. Several fragments were present in 0.5 M amounts while all others were 1.0 M no 0.25 M fragments were detected. Secondary restriction enzyme digestion of these isolated fragments with various enzymes, analysis of terminal fragments using both the methods of λ 5′ exonuclease digestion and end labeling with polynucleotide kinase, and blot hybridization experiments with 32P-labeled BamHI fragments indicated that this herpesvirus genome is a 92-Mdalton linear, double-stranded DNA molecule and is comprised of two segments designated as L (Long) and S (Short) which are 71.6 and 20.4 Mdalton, respectively. The 0.5 M fragments are located at the ends of the S region, an arrangement which allows the S region to invert relative to the L region; thus, two structural arrangements (isomers) of the genome exist. In addition, areas of heterogeneity were detected at the L terminus, within the S segment, and at a split variable locus in the L region.

Original languageEnglish (US)
Pages (from-to)97-114
Number of pages18
JournalVirology
Volume115
Issue number1
DOIs
StatePublished - 1981

Fingerprint

Equid Herpesvirus 1
Digestion
Genome
Enzymes
Polynucleotide 5'-Hydroxyl-Kinase
Phosphodiesterase I
Restriction Mapping
Herpesviridae
DNA
Molecular Structure

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Henry, B. E., Robinson, R. A., Dauenhauer, S. A., Atherton, S. S., Hayward, G. S., & O'Callaghan, D. J. (1981). Structure of the genome of equine herpesvirus type 1. Virology, 115(1), 97-114. https://doi.org/10.1016/0042-6822(81)90092-1

Structure of the genome of equine herpesvirus type 1. / Henry, Berch E.; Robinson, Robin A.; Dauenhauer, Steven A.; Atherton, Sally S.; Hayward, Gary Selwyn; O'Callaghan, Dennis J.

In: Virology, Vol. 115, No. 1, 1981, p. 97-114.

Research output: Contribution to journalArticle

Henry, BE, Robinson, RA, Dauenhauer, SA, Atherton, SS, Hayward, GS & O'Callaghan, DJ 1981, 'Structure of the genome of equine herpesvirus type 1', Virology, vol. 115, no. 1, pp. 97-114. https://doi.org/10.1016/0042-6822(81)90092-1
Henry BE, Robinson RA, Dauenhauer SA, Atherton SS, Hayward GS, O'Callaghan DJ. Structure of the genome of equine herpesvirus type 1. Virology. 1981;115(1):97-114. https://doi.org/10.1016/0042-6822(81)90092-1
Henry, Berch E. ; Robinson, Robin A. ; Dauenhauer, Steven A. ; Atherton, Sally S. ; Hayward, Gary Selwyn ; O'Callaghan, Dennis J. / Structure of the genome of equine herpesvirus type 1. In: Virology. 1981 ; Vol. 115, No. 1. pp. 97-114.
@article{28bf293353dc48eca2d24048f8b425e5,
title = "Structure of the genome of equine herpesvirus type 1",
abstract = "The molecular structure of the genome of equine herpesvirus type 1 (EHV-1) was determined by restriction endonuclease mapping studies. Primary restriction enzyme digestion of purified EHV-1 DNA, either unlabeled, 32P04 labeled, or [3H]TdR labeled, gave the following cleavage patterns: EcoRI yielded 17 fragments of 23.4 to 1.3 megadaltons (Mdalton); BglII, 16 fragments of 24.5 to 1.0 Mdalton; XbaI, 15 major fragments of 18.6 to 1.7 Mdalton; and BamHI,17 fragments of 13.7 to 2.8 Mdalton. Several fragments were present in 0.5 M amounts while all others were 1.0 M no 0.25 M fragments were detected. Secondary restriction enzyme digestion of these isolated fragments with various enzymes, analysis of terminal fragments using both the methods of λ 5′ exonuclease digestion and end labeling with polynucleotide kinase, and blot hybridization experiments with 32P-labeled BamHI fragments indicated that this herpesvirus genome is a 92-Mdalton linear, double-stranded DNA molecule and is comprised of two segments designated as L (Long) and S (Short) which are 71.6 and 20.4 Mdalton, respectively. The 0.5 M fragments are located at the ends of the S region, an arrangement which allows the S region to invert relative to the L region; thus, two structural arrangements (isomers) of the genome exist. In addition, areas of heterogeneity were detected at the L terminus, within the S segment, and at a split variable locus in the L region.",
author = "Henry, {Berch E.} and Robinson, {Robin A.} and Dauenhauer, {Steven A.} and Atherton, {Sally S.} and Hayward, {Gary Selwyn} and O'Callaghan, {Dennis J.}",
year = "1981",
doi = "10.1016/0042-6822(81)90092-1",
language = "English (US)",
volume = "115",
pages = "97--114",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Structure of the genome of equine herpesvirus type 1

AU - Henry, Berch E.

AU - Robinson, Robin A.

AU - Dauenhauer, Steven A.

AU - Atherton, Sally S.

AU - Hayward, Gary Selwyn

AU - O'Callaghan, Dennis J.

PY - 1981

Y1 - 1981

N2 - The molecular structure of the genome of equine herpesvirus type 1 (EHV-1) was determined by restriction endonuclease mapping studies. Primary restriction enzyme digestion of purified EHV-1 DNA, either unlabeled, 32P04 labeled, or [3H]TdR labeled, gave the following cleavage patterns: EcoRI yielded 17 fragments of 23.4 to 1.3 megadaltons (Mdalton); BglII, 16 fragments of 24.5 to 1.0 Mdalton; XbaI, 15 major fragments of 18.6 to 1.7 Mdalton; and BamHI,17 fragments of 13.7 to 2.8 Mdalton. Several fragments were present in 0.5 M amounts while all others were 1.0 M no 0.25 M fragments were detected. Secondary restriction enzyme digestion of these isolated fragments with various enzymes, analysis of terminal fragments using both the methods of λ 5′ exonuclease digestion and end labeling with polynucleotide kinase, and blot hybridization experiments with 32P-labeled BamHI fragments indicated that this herpesvirus genome is a 92-Mdalton linear, double-stranded DNA molecule and is comprised of two segments designated as L (Long) and S (Short) which are 71.6 and 20.4 Mdalton, respectively. The 0.5 M fragments are located at the ends of the S region, an arrangement which allows the S region to invert relative to the L region; thus, two structural arrangements (isomers) of the genome exist. In addition, areas of heterogeneity were detected at the L terminus, within the S segment, and at a split variable locus in the L region.

AB - The molecular structure of the genome of equine herpesvirus type 1 (EHV-1) was determined by restriction endonuclease mapping studies. Primary restriction enzyme digestion of purified EHV-1 DNA, either unlabeled, 32P04 labeled, or [3H]TdR labeled, gave the following cleavage patterns: EcoRI yielded 17 fragments of 23.4 to 1.3 megadaltons (Mdalton); BglII, 16 fragments of 24.5 to 1.0 Mdalton; XbaI, 15 major fragments of 18.6 to 1.7 Mdalton; and BamHI,17 fragments of 13.7 to 2.8 Mdalton. Several fragments were present in 0.5 M amounts while all others were 1.0 M no 0.25 M fragments were detected. Secondary restriction enzyme digestion of these isolated fragments with various enzymes, analysis of terminal fragments using both the methods of λ 5′ exonuclease digestion and end labeling with polynucleotide kinase, and blot hybridization experiments with 32P-labeled BamHI fragments indicated that this herpesvirus genome is a 92-Mdalton linear, double-stranded DNA molecule and is comprised of two segments designated as L (Long) and S (Short) which are 71.6 and 20.4 Mdalton, respectively. The 0.5 M fragments are located at the ends of the S region, an arrangement which allows the S region to invert relative to the L region; thus, two structural arrangements (isomers) of the genome exist. In addition, areas of heterogeneity were detected at the L terminus, within the S segment, and at a split variable locus in the L region.

UR - http://www.scopus.com/inward/record.url?scp=0019848716&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019848716&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(81)90092-1

DO - 10.1016/0042-6822(81)90092-1

M3 - Article

C2 - 6270904

AN - SCOPUS:0019848716

VL - 115

SP - 97

EP - 114

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -