Structure and specificity of antibody molecules.

R. J. Poljak, Mario L Amzel, B. L. Chen, R. P. Phizackerley, F. Saul

Research output: Contribution to journalArticle

Abstract

The structure of the Fab' fragment of a human myeloma protein (IgG1 (lambda) New) has been determined by X-ray crystallographic analysis to a nominal resolution of 0.2 nm. Each of the structure subunits corresponding to the variable and to the constant homology regions of the light and heavy polypeptide chains contains two irregular beta-sheets which are roughly parallel to each other and surround a tighly packed interior of hydrophobic side chains. The regions of the hypervariable sequences in the light and heavy chains occur in close spatial proximity at one end of the molecule, defining the active site of IgG New. The role of these hypervariable regions in defining the size and shape of the active site of different immunoglobulins is discussed on the basis of the three-dimensional model of Fab' New. Several ligands that bind to the active centre of IgG New have been used to obtain crystalline ligand-Fab' New complexes which were investigated by difference Fourier maps. These studies are analysed in terms of the biological function and specificity of antibodies.

Original languageEnglish (US)
Pages (from-to)43-51
Number of pages9
JournalPhilosophical Transactions of the Royal Society B: Biological Sciences
Volume272
Issue number915
StatePublished - Nov 6 1975
Externally publishedYes

Fingerprint

Antibody Specificity
active sites
antibody
Immunoglobulin G
myeloma
ligand
Molecules
Antibodies
Catalytic Domain
immunoglobulins
Ligands
Myeloma Proteins
Light
polypeptides
Immunoglobulin Fab Fragments
X-radiation
homology
X-ray spectroscopy
Immunoglobulins
X-Rays

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Poljak, R. J., Amzel, M. L., Chen, B. L., Phizackerley, R. P., & Saul, F. (1975). Structure and specificity of antibody molecules. Philosophical Transactions of the Royal Society B: Biological Sciences, 272(915), 43-51.

Structure and specificity of antibody molecules. / Poljak, R. J.; Amzel, Mario L; Chen, B. L.; Phizackerley, R. P.; Saul, F.

In: Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 272, No. 915, 06.11.1975, p. 43-51.

Research output: Contribution to journalArticle

Poljak, RJ, Amzel, ML, Chen, BL, Phizackerley, RP & Saul, F 1975, 'Structure and specificity of antibody molecules.', Philosophical Transactions of the Royal Society B: Biological Sciences, vol. 272, no. 915, pp. 43-51.
Poljak, R. J. ; Amzel, Mario L ; Chen, B. L. ; Phizackerley, R. P. ; Saul, F. / Structure and specificity of antibody molecules. In: Philosophical Transactions of the Royal Society B: Biological Sciences. 1975 ; Vol. 272, No. 915. pp. 43-51.
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