Structure and mechanism of DNA topoisomerase II

James M. Berger, Steven J. Gamblin, Stephen C. Harrison, James C. Wang

Research output: Contribution to journalArticlepeer-review

Abstract

The crystal structure of a large fragment of yeast type II DNA topoisomerase reveals a heart-shaped dimeric protein with a large central hole. It provides a molecular model of the enzyme as an ATP-modulated clamp with two sets of jaws at opposite ends, connected by multiple joints. An enzyme with bound DNA can admit a second DNA duplex through one set of jaws, transport it through the cleaved first duplex, and expel it through the other set of jaws.

Original languageEnglish (US)
Pages (from-to)225-232
Number of pages8
JournalNature
Volume379
Issue number6562
DOIs
StatePublished - Jan 18 1996
Externally publishedYes

ASJC Scopus subject areas

  • General

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