Structure and function of the adenovirus origin of replication

Dan R. Rawlins, Philip J. Rosenfeld, Ronald J. Wides, Mark D. Challberg, Thomas Kelly

Research output: Contribution to journalArticle

Abstract

Efficient initiation of adenovirus DNA replication requires the presence of specific terminal nucleotide sequences that collectively constitute the viral origin of replication. Using plasmids with deletions or base substitutions in a cloned segment of DNA derived from the terminus of the adenovirus 2 genome, we have demonstrated that the origin contains two functionally distinct regions. The first 18 bp of the viral genome are sufficient to support a limited degree of initiation. However, the presence of a sequence in the region between nucleotides 19 and 67 greatly enhances the efficiency of the initiation reaction. This region contains a specific binding site for a protein present in uninfected cells (KD = 2 × 10-11 M). The bound protein protects the DNA segment between base pairs 19 and 43 from attack by DNAase I. Studies with deletion mutants indicate that binding of the cellular protein is responsible for the enhancement of initiation.

Original languageEnglish (US)
Pages (from-to)309-319
Number of pages11
JournalCell
Volume37
Issue number1
DOIs
StatePublished - 1984

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Replication Origin
Adenoviridae
Viral Genome
Deoxyribonuclease I
DNA
Nucleotides
Genes
DNA Replication
Base Pairing
Carrier Proteins
Proteins
Plasmids
Binding Sites
Genome
Substitution reactions

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Rawlins, D. R., Rosenfeld, P. J., Wides, R. J., Challberg, M. D., & Kelly, T. (1984). Structure and function of the adenovirus origin of replication. Cell, 37(1), 309-319. https://doi.org/10.1016/0092-8674(84)90327-1

Structure and function of the adenovirus origin of replication. / Rawlins, Dan R.; Rosenfeld, Philip J.; Wides, Ronald J.; Challberg, Mark D.; Kelly, Thomas.

In: Cell, Vol. 37, No. 1, 1984, p. 309-319.

Research output: Contribution to journalArticle

Rawlins, DR, Rosenfeld, PJ, Wides, RJ, Challberg, MD & Kelly, T 1984, 'Structure and function of the adenovirus origin of replication', Cell, vol. 37, no. 1, pp. 309-319. https://doi.org/10.1016/0092-8674(84)90327-1
Rawlins, Dan R. ; Rosenfeld, Philip J. ; Wides, Ronald J. ; Challberg, Mark D. ; Kelly, Thomas. / Structure and function of the adenovirus origin of replication. In: Cell. 1984 ; Vol. 37, No. 1. pp. 309-319.
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