Structure and expression of germ line immunoglobulin heavychain ε transcripts: Interleukin-4 plus lipopolysaccharide-directed switching to Cε

Paul B Rothman, Yunn Y I Chen, Stuart Lutzker, C. L I Suzanne, Valerie Stewart, Robert Coffman, Frederick W. Alt

Research output: Contribution to journalArticle

Abstract

We have isolated cDNA clones complementary to a truncated immunoglobulin heavy-chain Cε RNA transcript previously found to be induced in B lymphoid cells by treatment with lipopolysaccharide (LPS) combined with interleukin-4 (IL-4). We demonstrate that this transcript initiates from a promoter upstream of the germ line ε class-switch recombination region (Sε region). The major germ line Cε transcript contains a small 5′ exon contributed by sequences upstream of the Sε region spliced to the normal Cε exons. Treatment of splenic B lymphoid cells with LPS plus IL-4 induces the expression of transcripts from the germ line ε transcription unit followed by expression of normal immunoglobulin ε heavy-chain mRNA. Furthermore, we demonstrate that similar treatment of transformed precursor B cell lines induces the expression of germ line ε transcripts followed by class switching to ε expression in these lines. This is the first demonstration of switching to ε in cells of the pre-B stage. The general structure of the germ line ε transcript and transcription unit is similar to that previously characterized for germ line γ2b transcripts. However, expression of these two germ line transcription units in B-lineage cells is inversely regulated by IL-4 (plus LPS) treatment, correlating with the effects of these treatments on switching to these loci.

Original languageEnglish (US)
Pages (from-to)1672-1679
Number of pages8
JournalMolecular and Cellular Biology
Volume10
Issue number4
StatePublished - 1990
Externally publishedYes

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Germ Cells
Interleukin-4
Lipopolysaccharides
Immunoglobulins
Immunoglobulin Heavy Chains
B-Lymphoid Precursor Cells
Genetic Recombination
Exons
Lymphocytes
Immunoglobulin Class Switching
Complementary DNA
Clone Cells
RNA
Cell Line
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Structure and expression of germ line immunoglobulin heavychain ε transcripts : Interleukin-4 plus lipopolysaccharide-directed switching to Cε. / Rothman, Paul B; Chen, Yunn Y I; Lutzker, Stuart; Suzanne, C. L I; Stewart, Valerie; Coffman, Robert; Alt, Frederick W.

In: Molecular and Cellular Biology, Vol. 10, No. 4, 1990, p. 1672-1679.

Research output: Contribution to journalArticle

Rothman, Paul B ; Chen, Yunn Y I ; Lutzker, Stuart ; Suzanne, C. L I ; Stewart, Valerie ; Coffman, Robert ; Alt, Frederick W. / Structure and expression of germ line immunoglobulin heavychain ε transcripts : Interleukin-4 plus lipopolysaccharide-directed switching to Cε. In: Molecular and Cellular Biology. 1990 ; Vol. 10, No. 4. pp. 1672-1679.
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AU - Stewart, Valerie

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AU - Alt, Frederick W.

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