Structure and dynamics of actin filament solutions in the presence of latrunculin A

Andre Palmer, Boyoung Cha, Denis Wirtz

Research output: Contribution to journalArticle

Abstract

We study the polymerization kinetics and linear rheology of actin filaments in the absence and in the presence of latrunculin A. Filamentous actin is a semiflexible polymer, and latrunculin A is an organic, actin-binding molecule. Using diffusing wave spectroscopy (DWS), we monitor the thermally excited motion of monodisperse polystyrene microspheres in semidilute solutions of actin filaments. From these measurements, we extract the microspheres mean-square displacement, which is related to the viscoelastic nature of the actin solutions. These optical measurements, along with mechanical measurements, suggest that despite its depolymerizing effect, latrunculin A promotes the strengthening of actin networks. DWS shows that while the scaling nature of the viscoelastic properties of actin filaments is essentially unmodified in the presence of latrunculin A at small time scales, the elasticity of actin solutions becomes enhanced for increasing latrunculin concentrations at large time scales. Complementary electron-microscopy measurements help uncover the structural origin of this enhanced elasticity at small time scales.

Original languageEnglish (US)
Pages (from-to)3007-3015
Number of pages9
JournalJournal of Polymer Science, Part B: Polymer Physics
Volume36
Issue number17
StatePublished - Dec 1998

Fingerprint

Actins
filaments
elastic properties
Microspheres
mechanical measurement
Elasticity
Spectroscopy
rheology
optical measurement
spectroscopy
monitors
polystyrene
electron microscopy
polymerization
Rheology
Electron microscopy
scaling
Polystyrenes
Polymerization
kinetics

Keywords

  • Actin
  • Diffusing-wave spectroscopy
  • Latrunculin
  • Rheology
  • Semiflexible polymer

ASJC Scopus subject areas

  • Materials Chemistry
  • Polymers and Plastics

Cite this

Structure and dynamics of actin filament solutions in the presence of latrunculin A. / Palmer, Andre; Cha, Boyoung; Wirtz, Denis.

In: Journal of Polymer Science, Part B: Polymer Physics, Vol. 36, No. 17, 12.1998, p. 3007-3015.

Research output: Contribution to journalArticle

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