Structurally distinct and stage-specific adenylyl cyclase genes play different roles in dictyostelium development

Geoffrey S. Pitt, Nina Milona, Jane Borleis, Kenneth C. Lin, Randall R Reed, Peter N Devreotes

Research output: Contribution to journalArticle

Abstract

We have isolated two adenylyl cyclase genes, designated ACA and ACG, from Dictyostelium. The proposed structure for ACA resembles that proposed for mammalian adenylyl cyclases: two large hydrophilic domains and two sets of six transmembrane spans. ACG has a novel structure, reminiscent of the membrane-bound guanylyl cyclases. An aca- mutant, created by gene disruption, has little detectable adenylyl cyclase activity and fails to aggregate, demonstrating that cAMP is required for cell-cell communication. cAMP is not required for motility, chemotaxis, growth, and cell division, which are unaffected. Constitutive expression in aca- cells of either ACA or ACG, which is normally expressed only during germination, restores aggregation and the ability to complete the developmental program. ACA expression restores receptor and guanine nucleotide-regulated adenylyl cyclase activity, while activity in cells expressing ACG is insensitive to these regulators. Although they lack ACA, which has a transporter-like structure, the cells expressing ACG secrete cAMP constitutively.

Original languageEnglish (US)
Pages (from-to)305-315
Number of pages11
JournalCell
Volume69
Issue number2
DOIs
StatePublished - Apr 17 1992

Fingerprint

Dictyostelium
Adenylyl Cyclases
Genes
Cells
Aptitude
Guanine Nucleotides
Guanylate Cyclase
Chemotaxis
Germination
Cell Communication
Cell Division
Agglomeration
Membranes
Communication
Growth

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Structurally distinct and stage-specific adenylyl cyclase genes play different roles in dictyostelium development. / Pitt, Geoffrey S.; Milona, Nina; Borleis, Jane; Lin, Kenneth C.; Reed, Randall R; Devreotes, Peter N.

In: Cell, Vol. 69, No. 2, 17.04.1992, p. 305-315.

Research output: Contribution to journalArticle

Pitt, Geoffrey S. ; Milona, Nina ; Borleis, Jane ; Lin, Kenneth C. ; Reed, Randall R ; Devreotes, Peter N. / Structurally distinct and stage-specific adenylyl cyclase genes play different roles in dictyostelium development. In: Cell. 1992 ; Vol. 69, No. 2. pp. 305-315.
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