Structurally distinct and stage-specific adenylyl cyclase genes play different roles in dictyostelium development

Geoffrey S. Pitt, Nina Milona, Jane Borleis, Kenneth C. Lin, Randall R. Reed, Peter N. Devreotes

Research output: Contribution to journalArticlepeer-review

Abstract

We have isolated two adenylyl cyclase genes, designated ACA and ACG, from Dictyostelium. The proposed structure for ACA resembles that proposed for mammalian adenylyl cyclases: two large hydrophilic domains and two sets of six transmembrane spans. ACG has a novel structure, reminiscent of the membrane-bound guanylyl cyclases. An aca- mutant, created by gene disruption, has little detectable adenylyl cyclase activity and fails to aggregate, demonstrating that cAMP is required for cell-cell communication. cAMP is not required for motility, chemotaxis, growth, and cell division, which are unaffected. Constitutive expression in aca- cells of either ACA or ACG, which is normally expressed only during germination, restores aggregation and the ability to complete the developmental program. ACA expression restores receptor and guanine nucleotide-regulated adenylyl cyclase activity, while activity in cells expressing ACG is insensitive to these regulators. Although they lack ACA, which has a transporter-like structure, the cells expressing ACG secrete cAMP constitutively.

Original languageEnglish (US)
Pages (from-to)305-315
Number of pages11
JournalCell
Volume69
Issue number2
DOIs
StatePublished - Apr 17 1992

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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