Structural variation of the mouse natural killer gene complex

D. A. Higuchi, Patrick Cahan, J. Gao, S. T. Ferris, J. Poursine-Laurent, T. A. Graubert, W. M. Yokoyama

Research output: Contribution to journalArticle

Abstract

The natural killer gene complex (NKC) on chromosome 6 contains clusters of genes that encode both activation and inhibitory receptors expressed on mouse natural killer (NK) cells. NKC genes, particularly belonging to the Nkrp1 and Ly49 gene families, display haplotype differences between different mouse strains and allelic polymorphisms of individual genes, as previously revealed by conventional analysis in a small number of inbred mouse strains. Herein we used array-based comparative genomic hybridization (aCGH) to efficiently compare the NKC in 21 mouse strains to the reference C57BL/6 strain. By using unsupervised clustering methods, we could sort these variations into the same groups as determined by previous RFLP analyses of Nkrp1 and Ly49 genes. Prospective analyses of aCGH and RFLP data validated these relationships. Moreover, aCGH data predicted monoclonal antibody reactivity with an allospecific determinant on molecules expressed by NK cells. Taken together, these data demonstrate the structural variation in the NKC between mouse strains as well as the usefulness of aCGH in analysis of complex, polymorphic gene clusters.

Original languageEnglish (US)
Pages (from-to)637-648
Number of pages12
JournalGenes and Immunity
Volume11
Issue number8
DOIs
StatePublished - Dec 2010
Externally publishedYes

Fingerprint

Comparative Genomic Hybridization
Genes
Multigene Family
Natural Killer Cells
Restriction Fragment Length Polymorphisms
Chromosomes, Human, Pair 6
Inbred Strains Mice
Haplotypes
Cluster Analysis
Monoclonal Antibodies

Keywords

  • array-based comparative genomic hybridization (aCGH)
  • natural killer (NK) cells
  • natural killer gene complex (NKC)
  • RFLPs

ASJC Scopus subject areas

  • Genetics(clinical)
  • Immunology
  • Genetics

Cite this

Higuchi, D. A., Cahan, P., Gao, J., Ferris, S. T., Poursine-Laurent, J., Graubert, T. A., & Yokoyama, W. M. (2010). Structural variation of the mouse natural killer gene complex. Genes and Immunity, 11(8), 637-648. https://doi.org/10.1038/gene.2010.48

Structural variation of the mouse natural killer gene complex. / Higuchi, D. A.; Cahan, Patrick; Gao, J.; Ferris, S. T.; Poursine-Laurent, J.; Graubert, T. A.; Yokoyama, W. M.

In: Genes and Immunity, Vol. 11, No. 8, 12.2010, p. 637-648.

Research output: Contribution to journalArticle

Higuchi, DA, Cahan, P, Gao, J, Ferris, ST, Poursine-Laurent, J, Graubert, TA & Yokoyama, WM 2010, 'Structural variation of the mouse natural killer gene complex', Genes and Immunity, vol. 11, no. 8, pp. 637-648. https://doi.org/10.1038/gene.2010.48
Higuchi DA, Cahan P, Gao J, Ferris ST, Poursine-Laurent J, Graubert TA et al. Structural variation of the mouse natural killer gene complex. Genes and Immunity. 2010 Dec;11(8):637-648. https://doi.org/10.1038/gene.2010.48
Higuchi, D. A. ; Cahan, Patrick ; Gao, J. ; Ferris, S. T. ; Poursine-Laurent, J. ; Graubert, T. A. ; Yokoyama, W. M. / Structural variation of the mouse natural killer gene complex. In: Genes and Immunity. 2010 ; Vol. 11, No. 8. pp. 637-648.
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