Structural proteins of oncogenic ribonucleic acid viruses. Interspec II, a new interspecies antigen

M. Strand, J. T. August

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Two Rauscher murine leukemia virus polypeptides with apparent molecular weights of 69,000 and 71,000 were purified and characterized. These polypeptides contain a previously undescribed interspecies antigenic determinant of mammalian oncogenic C type RNA viruses (interspec II), as was demonstrated by the reaction of the murine antigen with anti feline leukemia virus serum. Radioimmunoassay analysis showed that both polypeptides were precipitated by the anti feline virus serum, indicating that they each contained the interspecies determinant or that they were closely associated and coprecipitated. The interspec II antigen was distinguished from the known gs 3 interspecies antigen (interspec I) and the virus RNA dependent DNA polymerase by protein purification, physical properties, and immunologic analysis. The new antigen was not detected by competition radioimmunoassay in uninfected mouse cells or mouse cells productively infected with vesicular stomatitis virus.

Original languageEnglish (US)
Pages (from-to)5627-5633
Number of pages7
JournalJournal of Biological Chemistry
Issue number16
StatePublished - Dec 1 1973


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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