Structural insights into maize viviparous14, a key enzyme in the biosynthesis of the phytohormone abscisic acid

Simon A J Messing, Mario L Amzel, Sandra B Gabelli, Ignacia Echeverria, Jonathan T. Vogel, Jiahn Chou Guan, Bao Cai Tan, Harry J. Klee, Donald R. McCarty

Research output: Contribution to journalArticle

Abstract

The key regulatory step in the biosynthesis of abscisic acid (ABA), a hormone central to the regulation of several important processes in plants, is the oxidative cleavage of the 11,12 double bond of a 9-cis-epoxycarotenoid. The enzyme viviparous14 (VP14) performs this cleavage in maize (Zea mays), making it a target for the rational design of novel chemical agents and genetic modifications that improve plant behavior through the modulation of ABA levels. The structure of VP14, determined ° to 3.2-A resolution, provides both insight into the determinants of regio- and stereospecificity of this enzyme and suggests a possible mechanism for oxidative cleavage. Furthermore, mutagenesis of the distantly related CCD1 of maize shows how the VP14 structure represents a template for all plant carotenoid cleavage dioxygenases (CCDs). In addition, the structure suggests how VP14 associates with the membrane as a way of gaining access to its membrane soluble substrate.

Original languageEnglish (US)
Pages (from-to)2970-2980
Number of pages11
JournalPlant Cell
Volume22
Issue number9
DOIs
StatePublished - Sep 2010

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Abscisic Acid
Plant Growth Regulators
plant hormones
Zea mays
abscisic acid
biosynthesis
corn
Enzymes
enzymes
Dioxygenases
Membranes
Carotenoids
genetic engineering
mutagenesis
Mutagenesis
carotenoids
hormones
Hormones

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

Structural insights into maize viviparous14, a key enzyme in the biosynthesis of the phytohormone abscisic acid. / Messing, Simon A J; Amzel, Mario L; Gabelli, Sandra B; Echeverria, Ignacia; Vogel, Jonathan T.; Guan, Jiahn Chou; Tan, Bao Cai; Klee, Harry J.; McCarty, Donald R.

In: Plant Cell, Vol. 22, No. 9, 09.2010, p. 2970-2980.

Research output: Contribution to journalArticle

Messing, SAJ, Amzel, ML, Gabelli, SB, Echeverria, I, Vogel, JT, Guan, JC, Tan, BC, Klee, HJ & McCarty, DR 2010, 'Structural insights into maize viviparous14, a key enzyme in the biosynthesis of the phytohormone abscisic acid', Plant Cell, vol. 22, no. 9, pp. 2970-2980. https://doi.org/10.1105/tpc.110.074815
Messing, Simon A J ; Amzel, Mario L ; Gabelli, Sandra B ; Echeverria, Ignacia ; Vogel, Jonathan T. ; Guan, Jiahn Chou ; Tan, Bao Cai ; Klee, Harry J. ; McCarty, Donald R. / Structural insights into maize viviparous14, a key enzyme in the biosynthesis of the phytohormone abscisic acid. In: Plant Cell. 2010 ; Vol. 22, No. 9. pp. 2970-2980.
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