The Structural Genomics of Pathogenic Protozoa (SGPP) Consortium aimed to determine crystal structures of proteins from trypanosomatid and malaria parasites in a high throughput manner. The pipeline of target selection, protein production, crystallization, and structure determination, is sketched. Special emphasis is given to a number of technology developments including domain prediction, the use of "co-crystallants," and capillary crystallization. "Fragment cocktail crystallography" for medical structural genomics is also described.
|Original language||English (US)|
|Number of pages||17|
|Journal||Methods in molecular biology (Clifton, N.J.)|
|State||Published - 2008|
ASJC Scopus subject areas
- Molecular Biology