@article{78bdb3e6f47f42b28693369532d537a3,
title = "Structural diversity in the type IV Pili of multidrug-resistant acinetobacter",
abstract = "Acinetobacter baumannii is a Gram-negative coccobacillus found primarily in hospital settings that has recently emerged as a source of hospital-acquired infections. A. baumannii expresses a variety of virulence factors, including type IV pili, bacterial extracellular appendages often essential for attachment to host cells. Here, we report the high resolution structures of the major pilin subunit, PilA, from three Acinetobacter strains, demonstrating that A. baumannii subsets produce morphologically distinct type IV pilin glycoproteins. We examine the consequences of this heterogeneity for protein folding and assembly as well as host-cell adhesion by Acinetobacter. Comparisons of genomic and structural data with pilin proteins from other species of soil gammaproteobacteria suggest that these structural differences stem from evolutionary pressure that has resulted in three distinct classes of type IVa pilins, each found in multiple species.",
author = "Piepenbrink, {Kurt H.} and Erik Lillehoj and Harding, {Christian M.} and Labonte, {Jason W.} and Xiaotong Zuo and Rapp, {Chelsea A.} and Munson, {Robert S.} and Goldblum, {Simeon E.} and Feldman, {Mario F.} and Gray, {Jeffrey J.} and Sundberg, {Eric J.}",
note = "Funding Information: This work was supported in part by National Institutes of Health Grant R01 AI114902 (to E. J. S.). The authors declare that they have no conflicts of interest with the contents of this article. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Supported by National Institutes of Health Training Grant T32 AI095190 and National Institutes of Health Fellowship F32 AI110045. Supported by National Institutes of Health Fellowship F32 CA189246. Acknowledgments-We thank the staff at Argonne National Laboratory Advanced Photon Source, General Medical Sciences and Cancer Institutes Structural Biology Facility, beam lines 23ID-D and 23ID-B, and the staff at Stanford Synchrotron Radiation Lightsource, beam line 12-2, for technical assistance with x-ray data collection. We also thank Dr. Angela Wilks for the use of the circular dichroism spectrophotometer.",
year = "2016",
month = oct,
day = "28",
doi = "10.1074/jbc.M116.751099",
language = "English (US)",
volume = "291",
pages = "22924--22935",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "44",
}