Structural determination of lipid a from gram negative bacteria using laser desorption mass spectrometry

Robert J. Cotter, Jeffrey Honovich, Nilofer Qureshi, Kuni Takayama

Research output: Contribution to journalArticle


Laser desorption mass spectrometry has been employed for the structural determination of lipid A components derived from the lipopolysaccharides (LPS) of gram‐negative bacteria. Mass spectra were obtained for methylated monophosphoryl lipid A from Neisseria gonorrhoeae and Rhodopseudomonas sphaeroides, for diphosphoryl lipid A from Escherichia coli and for the intact LPS from the Re Mutant of E. coli consisting of triphosphoryl lipid A and two KDO (2‐keto‐3‐deoxyoctonate) units. Fragmentation of the phosphate (or pyrophosphate) on the reducing glucosamine is followed by fragmentation of acyl‐linked fatty acids. Also observed are fragmentions which correspond to the distal portion of the molecule.

Original languageEnglish (US)
Pages (from-to)591-598
Number of pages8
JournalBiomedical & Environmental Mass Spectrometry
Issue number11
StatePublished - Nov 1987


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Pollution

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