Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction

Adelaide U P Hain, Ryan R. Weltzer, Holly Hammond, Bamini Jayabalasingham, Rhoel R. Dinglasan, David Graham, David R. Colquhoun, Isabelle Coppens, Jürgen Bosch

Research output: Contribution to journalArticle

Abstract

The autophagy-related proteins are thought to serve multiple functions in Plasmodium and are considered essential to parasite survival and development. We have studied two key interacting proteins, Atg8 and Atg3, of the autophagy pathway in Plasmodium falciparum. These proteins are vital for the formation and elongation of the autophagosome and essential to the process of macroautophagy. Autophagy may be required for conversion of the sporozoite into erythrocytic-infective merozoites and may be crucial for other functions during asexual blood stages. Here we describe the identification of an Atg8 family interacting motif (AIM) in Plasmodium Atg3, which binds Plasmodium Atg8. We determined the co-crystal structure of PfAtg8 with a short Atg3103-110 peptide, corresponding to this motif, to 2.2å resolution. Our in vitro interaction studies are in agreement with our X-ray crystal structure. Furthermore they suggest an important role for a unique Apicomplexan loop absent from human Atg8 homologues. Prevention of the protein-protein interaction of full length PfAtg8 with PfAtg3 was achieved at low micromolar concentrations with a small molecule, 1,2,3-trihydroxybenzene. Together our structural and interaction studies represent a starting point for future antimalarial drug discovery and design for this novel protein-protein interaction.

Original languageEnglish (US)
Pages (from-to)551-562
Number of pages12
JournalJournal of Structural Biology
Volume180
Issue number3
DOIs
StatePublished - Dec 2012

Fingerprint

Plasmodium
Autophagy
Proteins
Pyrogallol
Merozoites
Sporozoites
Drug Design
Antimalarials
Drug Discovery
Plasmodium falciparum
Parasites
X-Rays
Peptides

Keywords

  • Autophagy
  • Fragment library
  • Fragment-based drug discovery
  • Malaria
  • Plasmodium
  • Protein-protein interaction
  • Surface plasmon resonance
  • X-ray crystallography

ASJC Scopus subject areas

  • Structural Biology

Cite this

Hain, A. U. P., Weltzer, R. R., Hammond, H., Jayabalasingham, B., Dinglasan, R. R., Graham, D., ... Bosch, J. (2012). Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. Journal of Structural Biology, 180(3), 551-562. https://doi.org/10.1016/j.jsb.2012.09.001

Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. / Hain, Adelaide U P; Weltzer, Ryan R.; Hammond, Holly; Jayabalasingham, Bamini; Dinglasan, Rhoel R.; Graham, David; Colquhoun, David R.; Coppens, Isabelle; Bosch, Jürgen.

In: Journal of Structural Biology, Vol. 180, No. 3, 12.2012, p. 551-562.

Research output: Contribution to journalArticle

Hain, AUP, Weltzer, RR, Hammond, H, Jayabalasingham, B, Dinglasan, RR, Graham, D, Colquhoun, DR, Coppens, I & Bosch, J 2012, 'Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction', Journal of Structural Biology, vol. 180, no. 3, pp. 551-562. https://doi.org/10.1016/j.jsb.2012.09.001
Hain, Adelaide U P ; Weltzer, Ryan R. ; Hammond, Holly ; Jayabalasingham, Bamini ; Dinglasan, Rhoel R. ; Graham, David ; Colquhoun, David R. ; Coppens, Isabelle ; Bosch, Jürgen. / Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. In: Journal of Structural Biology. 2012 ; Vol. 180, No. 3. pp. 551-562.
@article{247d9f0da94f402a8cfd14f874f2f7cd,
title = "Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction",
abstract = "The autophagy-related proteins are thought to serve multiple functions in Plasmodium and are considered essential to parasite survival and development. We have studied two key interacting proteins, Atg8 and Atg3, of the autophagy pathway in Plasmodium falciparum. These proteins are vital for the formation and elongation of the autophagosome and essential to the process of macroautophagy. Autophagy may be required for conversion of the sporozoite into erythrocytic-infective merozoites and may be crucial for other functions during asexual blood stages. Here we describe the identification of an Atg8 family interacting motif (AIM) in Plasmodium Atg3, which binds Plasmodium Atg8. We determined the co-crystal structure of PfAtg8 with a short Atg3103-110 peptide, corresponding to this motif, to 2.2{\aa} resolution. Our in vitro interaction studies are in agreement with our X-ray crystal structure. Furthermore they suggest an important role for a unique Apicomplexan loop absent from human Atg8 homologues. Prevention of the protein-protein interaction of full length PfAtg8 with PfAtg3 was achieved at low micromolar concentrations with a small molecule, 1,2,3-trihydroxybenzene. Together our structural and interaction studies represent a starting point for future antimalarial drug discovery and design for this novel protein-protein interaction.",
keywords = "Autophagy, Fragment library, Fragment-based drug discovery, Malaria, Plasmodium, Protein-protein interaction, Surface plasmon resonance, X-ray crystallography",
author = "Hain, {Adelaide U P} and Weltzer, {Ryan R.} and Holly Hammond and Bamini Jayabalasingham and Dinglasan, {Rhoel R.} and David Graham and Colquhoun, {David R.} and Isabelle Coppens and J{\"u}rgen Bosch",
year = "2012",
month = "12",
doi = "10.1016/j.jsb.2012.09.001",
language = "English (US)",
volume = "180",
pages = "551--562",
journal = "Journal of Structural Biology",
issn = "1047-8477",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction

AU - Hain, Adelaide U P

AU - Weltzer, Ryan R.

AU - Hammond, Holly

AU - Jayabalasingham, Bamini

AU - Dinglasan, Rhoel R.

AU - Graham, David

AU - Colquhoun, David R.

AU - Coppens, Isabelle

AU - Bosch, Jürgen

PY - 2012/12

Y1 - 2012/12

N2 - The autophagy-related proteins are thought to serve multiple functions in Plasmodium and are considered essential to parasite survival and development. We have studied two key interacting proteins, Atg8 and Atg3, of the autophagy pathway in Plasmodium falciparum. These proteins are vital for the formation and elongation of the autophagosome and essential to the process of macroautophagy. Autophagy may be required for conversion of the sporozoite into erythrocytic-infective merozoites and may be crucial for other functions during asexual blood stages. Here we describe the identification of an Atg8 family interacting motif (AIM) in Plasmodium Atg3, which binds Plasmodium Atg8. We determined the co-crystal structure of PfAtg8 with a short Atg3103-110 peptide, corresponding to this motif, to 2.2å resolution. Our in vitro interaction studies are in agreement with our X-ray crystal structure. Furthermore they suggest an important role for a unique Apicomplexan loop absent from human Atg8 homologues. Prevention of the protein-protein interaction of full length PfAtg8 with PfAtg3 was achieved at low micromolar concentrations with a small molecule, 1,2,3-trihydroxybenzene. Together our structural and interaction studies represent a starting point for future antimalarial drug discovery and design for this novel protein-protein interaction.

AB - The autophagy-related proteins are thought to serve multiple functions in Plasmodium and are considered essential to parasite survival and development. We have studied two key interacting proteins, Atg8 and Atg3, of the autophagy pathway in Plasmodium falciparum. These proteins are vital for the formation and elongation of the autophagosome and essential to the process of macroautophagy. Autophagy may be required for conversion of the sporozoite into erythrocytic-infective merozoites and may be crucial for other functions during asexual blood stages. Here we describe the identification of an Atg8 family interacting motif (AIM) in Plasmodium Atg3, which binds Plasmodium Atg8. We determined the co-crystal structure of PfAtg8 with a short Atg3103-110 peptide, corresponding to this motif, to 2.2å resolution. Our in vitro interaction studies are in agreement with our X-ray crystal structure. Furthermore they suggest an important role for a unique Apicomplexan loop absent from human Atg8 homologues. Prevention of the protein-protein interaction of full length PfAtg8 with PfAtg3 was achieved at low micromolar concentrations with a small molecule, 1,2,3-trihydroxybenzene. Together our structural and interaction studies represent a starting point for future antimalarial drug discovery and design for this novel protein-protein interaction.

KW - Autophagy

KW - Fragment library

KW - Fragment-based drug discovery

KW - Malaria

KW - Plasmodium

KW - Protein-protein interaction

KW - Surface plasmon resonance

KW - X-ray crystallography

UR - http://www.scopus.com/inward/record.url?scp=84868506702&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84868506702&partnerID=8YFLogxK

U2 - 10.1016/j.jsb.2012.09.001

DO - 10.1016/j.jsb.2012.09.001

M3 - Article

C2 - 22982544

AN - SCOPUS:84868506702

VL - 180

SP - 551

EP - 562

JO - Journal of Structural Biology

JF - Journal of Structural Biology

SN - 1047-8477

IS - 3

ER -